Silane PEG Biotin

Synonym: Silane PEG Biotin, Biotin PEG Silane, Silane polyethylene glycol biotin, Biotin polyethylene glycol Silane

Description:

Ethoxy or methoxyl silane functionalized polyethylene glycol Biotin, silane PEG Biotin (Biotin-PEG-Si) is one of Nanocs surface reactive PEG derivatives that can be used to modify glass, silica and other surfaces via the reaction between hydroxyl group and ethoxyl/methoxyl silane. Biotin functional silane PEG is able to attached polyethylene glycol to various glass or metal surface biotin tag. Pegylation can greatly suppress the non-specific binding of charged molecules to the modified surfaces. And they have wide applications for medica device, biomems or biocompatible material development.

Physical Properties:

• Form: Solid
• Color: White
• Reactive group: Triethoxysilane
• Functional tag: Biotin
• Solubility: Soluble in water
• Purity: >95%
• Applications: Surface modification

Storage Conditions:

• Store at -20 ⁰C. Desiccate. Silane PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen. USE ASAP.

Reaction Procedures:

Generally, silane PEG is first dissolved in a mixture of ethanol/water  or DMSO solution, then allow the solution to contact with oxidized glass or silica surface for 30 min to 2 hours. Under this condition, silane PEG should bind to the material covalently.

Materials Required:

• Pegylation solution: Ethanol/water (w/w, 95%/5%);
• Silane PEG stock solution, 10~50 mg/mL in pegylation solution, prepare in fresh.
• Washing solution: Distilled water.

Reaction Steps:

1. Dissolve targeted materials in Pegylation buffer.
2. Add silane PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of silane PEG needed for optimal conjugation;
3. Allow mixture agitates at room temperature for 30 min at room temperature or 2 hours at 4 -20 ⁰C.
4. Wash out unreacted materials either by distilled water.

References:

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