Okadaic acid(Synonyms: 冈田酸)

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Okadaic acid (Synonyms: 冈田酸) 纯度: ≥98.0%

Okadaic acid 是一种海洋毒素，是蛋白质磷酸酶 (PP) 的抑制剂，包括 PP1 (IC50=15-50 nM)、PP2A (IC50=0.1-0.3 nM)、PP3 (IC50=3.7-4 nM)、PP4 (IC50=0.1 nM) 和 PP5 (IC50=3.5 nM)，其中对 PP2A 的亲和力最显著，但不抑制 PP2C。Okadaic acid 通过抑制 PP 增加了多种蛋白的磷酸化，并具有肿瘤启动子的作用。Okadaic acid 诱导 tau 蛋白磷酸化。

Okadaic acid(Synonyms: 冈田酸)

Okadaic acid Chemical Structure

CAS No. : 78111-17-8

规格 价格 是否有货 数量
25 μg (124.2 μM * 250 μL in Ethanol) ￥2300 In-stock

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生物活性

Okadaic acid, a marine toxin, is an inhibitor of protein phosphatases (PP). Okadaic acid has a significantly higher affinity for PP2A (IC50=0.1-0.3 nM), and inhibits PP1 (IC50=15-50 nM), PP3 (IC50=3.7-4 nM), PP4 (IC50=0.1 nM), PP5 (IC50=3.5 nM), but does not inhibit PP2C. Okadaic acid increases of phosphorylation of a number of proteins by inhibiting PP, and acts a tumor promoter. Okadaic acid induces tau phosphorylation[1][2].

IC50 & Target[1]

PP1

15-50 nM (IC50)

PP2A

0.1-0.3 nM (IC50)

PP3

3.7-4 nM (IC50)

PP4

0.1 nM (IC50)

PP5

3.5 nM (IC50)

PP2B

~4000 nM (IC50)

PP7

＞1000 nM (IC50)

体外研究
(In Vitro)

Okadaic acid (0-100 nM; 24 h or 48 h) inhibits the proliferation of AGS, MNK-45, Caco 2 cells[3].
Okadaic acid (10 nM; 8 hours) increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons[4].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[3]

Cell Line: AGS, MNK-45 and Caco 2 cell lines
Concentration: 0-100 nM
Incubation Time: 24 h or 48 h
Result: Inhibited the proliferation of AGS, MNK-45, Caco 2 cells.

体内研究
(In Vivo)

Okadaic acid (100 μM; injected unilaterally to the lateral amygdala) induces Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection[5].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

805.00

Formula

C44H68O13
CAS 号

78111-17-8
中文名称

冈田酸
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Solution, -20°C, 2 years
参考文献

  • [1]. Kleppe R, et al. Cell Death Inducing Microbial Protein Phosphatase Inhibitors–Mechanisms of Action. Mar Drugs. 2015 Oct 22;13(10):6505-20.

    [2]. Valdiglesias V, et al. Okadaic acid: more than a diarrheic toxin. Mar Drugs. 2013 Oct 31;11(11):4328-49.

    [3]. del Campo M, et al. Okadaic acid toxin at sublethal dose produced cell proliferation in gastric and colon epithelial cell lines. Mar Drugs. 2013;11(12):4751-4760.

    [4]. Cho MH, et al. Increased phosphorylation of dynamin-related protein 1 and mitochondrial fission in okadaic acid-treated neurons. Brain Res. 2012 May 15;1454:100-10.

    [5]. Baker S, et al. A local insult of okadaic acid in wild-type mice induces tau phosphorylation and protein aggregation in anatomically distinct brain regions. Acta Neuropathol Commun. 2016;4:32.

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