5′-Methylthioadenosine(Synonyms: 5′-(Methylthio)-5′-deoxyadenosine; 5′-Deoxy-5′-(methylthio)adenosine; 5′-S-Methyl-5′-thioadenosine)

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5′-Methylthioadenosine (Synonyms: 5′-(Methylthio)-5′-deoxyadenosine; 5′-Deoxy-5′-(methylthio)adenosine; 5′-S-Methyl-5′-thioadenosine) 纯度: 99.67%

5′-Methylthioadenosine (5′-(Methylthio)-5′-deoxyadenosine) 是由 S-腺苷蛋氨酸 (SAM) 在多胺合成过程中生成的核苷。5′-Methylthioadenosine 通过抑制肿瘤细胞增殖、侵袭和诱导细胞凋亡 (apoptosis) 来抑制肿瘤，同时控制肿瘤组织的炎症微环境。5′-Methylthioadenosine 及其相关化合物对肿瘤发生具有显着的调节作用。

5

5′-Methylthioadenosine Chemical Structure

CAS No. : 2457-80-9

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥550 In-stock
25 mg ￥500 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

5′-Methylthioadenosine 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Human Endogenous Metabolite Compound Library
  • Nucleotide Compound Library
  • Alkaloids Library
  • Gut Microbial Metabolite Library
  • Microbial Metabolite Library
  • Food-Sourced Compound Library

生物活性

5′-Methylthioadenosine (5′-(Methylthio)-5′-deoxyadenosine) is a nucleoside generated from S-adenosylmethionine (SAM) during polyamine synthesis[1]. 5′-Methylthioadenosine suppresses tumors by inhibiting tumor cell proliferation, invasion, and the induction of apoptosis while controlling the inflammatory micro-environments of tumor tissue. 5′-Methylthioadenosine and its associated materials have striking regulatory effects on tumorigenesis[2].

IC50 & Target

Human Endogenous Metabolite

 

Microbial Metabolite

 

体外研究
(In Vitro)

5′-Methylthioadenosine protects MTAP+ cells significantly better than MTAP− cells from 6TG (6′-thioguanine) toxicity[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: MTAP+ and MTAP− NIH3T3 cells, isogenic MTAP+ and MTAP− cell line derived from a human HT1080 fibrosarcoma cell line
Concentration: 6TG and 2FA in combination with 10 μM MTA
Incubation Time: 48 hours
Result: Significantly increased IC50 concentration in MTAP+ cells, but no significant change in MTAP− cells.

体内研究
(In Vivo)

The 2FA (2′-fluoroadenine) + MTA (5′-Methylthioadenosine) combination (100 mg/kg MTA; 20 mg/kg 2FA) inhibits tumor growth of four different MTAP− human tumor cell lines in mouse xenograft models[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

297.33

Formula

C11H15N5O3S
CAS 号

2457-80-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

DMSO : 50 mg/mL (168.16 mM; Need ultrasonic)

DMF : 2.5 mg/mL (8.41 mM; Need ultrasonic)

H2O : 1 mg/mL (3.36 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3633 mL 16.8163 mL 33.6327 mL
5 mM 0.6727 mL 3.3633 mL 6.7265 mL
10 mM 0.3363 mL 1.6816 mL 3.3633 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.41 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.41 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.41 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Tang Y, et al. 5′-Methylthioadenosine attenuates ischemia reperfusion injury after liver transplantation in rats. Inflammation. 2014;37(5):1366-1373.

    [2]. Li Y, et al. 5′-Methylthioadenosine and Cancer: old molecules, new understanding. J Cancer. 2019;10(4):927-936.

    [3]. Tang B, et al. Specific Targeting of MTAP-Deleted Tumors with a Combination of 2′-Fluoroadenine and 5′-Methylthioadenosine. Cancer Res. 2018;78(15):4386-4395.

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