Eicosapentaenoic Acid(Synonyms: 二十碳五烯酸; EPA; Timnodonic acid)

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Eicosapentaenoic Acid (Synonyms: 二十碳五烯酸; EPA; Timnodonic acid) 纯度: ≥95.0%

Eicosapentaenoic Acid (EPA) 是一种具有口服活性的 ω-3 长链多不饱和脂肪酸 (ω-3 LC-PUFAs)。Eicosapentaenoic Acid 具有促进肿瘤抑制基因 CCAAT/增强子结合蛋白δ (C/EBPδ) 重新表达的 DNA 去甲基化作用。Eicosapentaenoic Acid 通过 H-RAS 内含子 1 CpG 岛去甲基化激活U937 白血病细胞中的 RAS/ERK/C/EBPβ 途径。Eicosapentaenoic Acid 能促进血管平滑肌细胞的松弛和血管舒张。

Eicosapentaenoic Acid(Synonyms: 二十碳五烯酸; EPA;  Timnodonic acid)

Eicosapentaenoic Acid Chemical Structure

CAS No. : 10417-94-4

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥605 In-stock
50 mg ¥550 In-stock
100 mg ¥750 In-stock
500 mg ¥3000 In-stock
1 g   询价  
5 g   询价  

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生物活性

Eicosapentaenoic Acid (EPA) is an orally active Omega-3 long-chain polyunsaturated fatty acid (ω-3 LC-PUFA). Eicosapentaenoic Acid exhibits a DNA demethylating action that promotes the re-expression of the tumor suppressor gene CCAAT/enhancer-binding protein δ (C/EBPδ). Eicosapentaenoic Acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells. Eicosapentaenoic Acid can promote relaxation of vascular smooth muscle cells and vasodilation[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

C/EBPβ phosphorylated form is evident in Eicosapentaenoic Acid (EPA; 100 µM; 24 h) treated cells, whereas it was barely detectable in control, and OA or LA treated U937[1].
Eicosapentaenoic Acid (100 µM; 1, 3, 24 h) causes a significant increase of H-Ras and N-Ras mRNA levels conditioning for 1 and 3 hours. K-Ras mRNA levels were not affected by Eicosapentaenoic Acid[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

302.45

Formula

C20H30O2

CAS 号

10417-94-4

中文名称

二十碳五烯酸

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

Ethanol : ≥ 100 mg/mL (330.63 mM)

DMSO : ≥ 53 mg/mL (175.24 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3063 mL 16.5317 mL 33.0633 mL
5 mM 0.6613 mL 3.3063 mL 6.6127 mL
10 mM 0.3306 mL 1.6532 mL 3.3063 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% EtOH    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.27 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中,混合均匀。

  • 2.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.08 mg/mL (6.88 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (6.88 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (6.88 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (6.88 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 4.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.88 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.88 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献
  • [1]. Martins, J.G., EPA but not DHA appears to be responsible for the efficacy of omega-3 long chain polyunsaturated fatty acid supplementation in depression: evidence from a meta-analysis of randomized controlled trials. J Am Coll Nutr, 2009. 28(5): p. 525-42.

    [2]. Miaozhen Pan, et al. Dietary ω-3 polyunsaturated fatty acids are protective for myopia. Proc Natl Acad Sci U S A. 2021 Oct 26;118(43):e2104689118.

    [3]. Shun-he Wang, et al. Endogenous omega-3 long-chain fatty acid biosynthesis from alpha-linolenic acid is affected by substrate levels, gene expression, and product inhibition. RSC Adv., 2017, 7, 40946-40951.

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