Acetylcholine chloride(Synonyms: 氯化乙酰胆碱; ACh chloride)

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Acetylcholine chloride (Synonyms: 氯化乙酰胆碱; ACh chloride) 纯度: ≥98.0%

Acetylcholine chloride (ACh chloride) 是一种神经递质，是一种有效的胆碱能 (cholinergic) 激动剂。Acetylcholine chloride 是通过刺激烟碱乙酰胆碱受体 (nAChRs) 调节多巴胺能 (DAergic) 神经元活性。Acetylcholine chloride 在体外抑制 p53 突变肽的聚集。

Acetylcholine chloride(Synonyms: 氯化乙酰胆碱; ACh chloride)

Acetylcholine chloride Chemical Structure

CAS No. : 60-31-1

规格 价格 是否有货 数量
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10 mM * 1 mL in DMSO ￥550 In-stock
500 mg ￥500 In-stock
1 g ￥600 In-stock
5 g ￥900 In-stock
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生物活性

Acetylcholine chloride (ACh chloride), a neurotransmitter, is a potent cholinergic agonist. Acetylcholine chloride is a modulator of the activity of dopaminergic (DAergic) neurons through the stimulation of nicotinic acetylcholine receptors (nAChRs)[1][2]. Acetylcholine chloride inhibits p53 mutant peptide aggregation in vitro[5].

IC50 & Target[1][5]

Ca2+

 

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Acetylcholine chloride (ACh chloride; 10 μM) opens the calcium channel and the fluorescence value and intracellular free calcium will increase significantly when the medium is with high calcium, while these will decrease when the medium is without calcium in sweat gland epithelial cells[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Acetylcholine chloride (ACh chloride; SC; 20 mg/kg; single dose) induces pronounced cholinergic stimulation and increase of mouse survival in experimental infection[4].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male and female out-bred albino mice weighing 18-22 g (sepsis)[4]
Dosage: 20 mg/kg
Administration: SC; single dose
Result: Significantly reduces mortality of mice from sepsis induced by intraperitoneal injection of 2×109 E. coli bacterial bodies and the blood levels of proinflammatory cytokines TNF-α, IL-1β, and IL-6.

Clinical Trial

分子量

181.66

Formula

C7H16ClNO2
CAS 号

60-31-1
中文名称

氯化乙酰胆碱
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro: 

H2O : 100 mg/mL (550.48 mM; Need ultrasonic)

DMSO : 62.5 mg/mL (344.05 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.5048 mL 27.5239 mL 55.0479 mL
5 mM 1.1010 mL 5.5048 mL 11.0096 mL
10 mM 0.5505 mL 2.7524 mL 5.5048 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： PBS

    Solubility: 23.33 mg/mL (128.43 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (11.45 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (11.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (11.45 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (11.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 4.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (11.45 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (11.45 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Prashant Tiwari, et al. Basic and modern concepts on cholinergic receptor: A review. Asian Pac J Trop Dis. 2013 Oct;3(5): 413-420.

    [2]. A Young, et al. Diarrhoea of famine and malnutrition–investigations using a rat model. 2–Ileal hypersecretion induced by starvation. Gut. 1990 Feb;31(2):162-9.

    [3]. Xia Lei, et al. Effects of acetylcholine chloride on intracellular calcium concentration of cultured sweat gland epithelial cells. Arch Dermatol Res. 2008 Aug;300(7):335-41.

    [4]. P F Zabrodskii, et al. Effect of acetylcholine on mortality of mice from sepsis and proinflammatory cytokine production. Bull Exp Biol Med. 2011 Jan;150(3):340-2.

    [5]. Zhaolin Chen, et al. Inhibition of p53 Mutant Peptide Aggregation In Vitro by Cationic Osmolyte Acetylcholine Chloride. Protein Pept Lett. 2017;24(4):353-357.

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