HT 培养基补充剂 (50×) Hybri-Max™

应用

Ht培养基补充剂是优质的次黄嘌呤和胸腺嘧啶混合物，可用于杂交瘤筛选和细胞培养应用。HT培养基补充剂 (50×) Hybri-Max™已用作INS-1 β-细胞共培养基的补充剂。 这一成分适合用作杂交瘤细胞培养基以及HeLa S3和MTH1-KO细胞的杜氏改良Eagle培养基（DMEM）。

适用性

原理

制备说明

外形

重悬

10mL 无菌细胞培养基可复原一小瓶本品。储存液足以制备 500mL 培养基。最终工作浓度：100μM 次黄嘌呤、16μM 胸腺嘧啶核苷。

组分

Description

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max™has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.Reconstitution: Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.