制霉菌素即用型溶液

一般描述

制霉菌素是多烯大环内酯类抗真菌药，它是由 诺尔斯氏链霉菌 ATCC 11455 生产的聚酮化合物。该抗生素含有一个大内酯环，其天然合成是通过乙酰辅酶 A 起始的，然后缩合三个乙基丙二酰辅酶 A 和 15 个丙二酰辅酶 A 延伸部分。 化学结构：多烯

应用

制霉菌素已用于- 处理人巨噬细胞 U937，研究制霉菌素对银纳米颗粒处理的 U937 细胞系产生 IL-8（白介素8）的影响 确定其对 COS-7 细胞系中 SR-BI（B 类清道夫受体）介导的 OxLDL（氧化的低密度脂蛋白）细胞内积累的影响 作为小鼠巨噬细胞中的脂质巨噬细胞调节剂，研究脂质筏数目增加与 TNF-α（肿瘤坏死因子）应答增强之间的关系 制霉菌素是一种多烯类抗生素。 它的用途包括防止细胞培养物污染，并在表达 TLR2 的 THP1 细胞中诱导白介素（IL）-1、IL-8 和肿瘤坏死因子 α 分泌。 该产品的推荐细胞培养使用浓度为50 mg/L。

其他说明

保存于密封容器内，置于干燥通风处。在惰性气体下储存。对空气和光敏感。置于干燥处保存。

制备说明

在28°C，制霉菌素可溶于甲醇（11.2 mg/mL）、乙醇（1.2 mg/mL）、四氯化碳（1.23 mg/mL）、氯仿（0.48 mg/mL）、苯（0.28 mg/mL）和乙二醇（8.75 mg/mL）。 也可以5 mg/mL溶于DMSO，易溶于DMF和甲酰胺。 不建议对制霉菌素溶液进行高压灭菌或无菌过滤。 制霉菌素混悬液也有效，37°C下可稳定保存3天。 制备在水中的 50 mg/mL 悬浮液，并储存于 -20°C。

General Description

Chemical structure: polyene Nystatin is a polyene macrolide antifungal, which is a polyketide produced by Streptomyces noursei ATCC 11455. This antibiotic contains a macrolactone ring, and its natural synthesis is initiated with acetyl CoA, which is followed by the condensation of three ethylmalonyl-CoA and 15 malonyl-CoA extender moieties.

Application

Nystatin has been used – for the treatment of human macrophage cell line U937, to study the effect of nystatin on the production of IL-8 (interleukin 8) by silver nanoparticle-treated U937 cell line to determine its effect on intracellular accumulation of OxLDL (oxidized low density lipoprotein) mediated by SR-BI (scavenger receptor class B type I) in COS-7 cell line as a lipid raft modulator on mice macrophages to study the relationship between increased number of lipid rafts and the heightened response of TNF-α (tumor necrosis factor) Nystatin is a polyene antibiotic. Its uses include preventing cell culture contamination and inducing interleukin (IL)-1, IL-8, and tumor necrosis factor α secretion in TLR2-expressing THP1 cells. This product is recommended for use in cell culture applications at 50 mg/L.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Store under inert gas. Air and light sensitive. Keep in a dry place.