来自化脓性链球菌的链球菌溶血素O
基本信息
| 产品名称 | 来自化脓性链球菌的链球菌溶血素O |
|---|---|
| 英文名称 | Streptolysin O from Streptococcus pyogenes |
| 规格或纯度 | 冷冻干燥粉末,蛋白质含量约3%,由Lowry提供,25000-50000 U/瓶 |
| 生化机理 | 可透过动物细胞膜、由巯基活化的毒素。该蛋白作为单体与膜胆固醇结合，随后聚合成 >12 nm 孔隙周围的大弧形和环形结构。Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass. It is thiol-activated. It is inhibited by allicin, an active component of garlic. |
一般描述
Description
Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs.Streptolysin O is a toxin secreted byStreptococcus pyogenesand is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy.Permeabilizes membranes to permit cellular uptake of large or charged molecules.
Preparation instructions
Form
Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.
Principle
相关属性
| CAS编号 | 98072-47-0 |
|---|---|
| 溶解性 | H2O：可溶 1mg/mL |
| 储存温度 | 干燥 |
| MDL号 | MFCD00132389 |
| 品牌 | Jinpan |
| PubChem CID | 135280160 |