GRF (1-29) amide (rat)(Synonyms: rGHRH(1-29)NH2)

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GRF (1-29) amide (rat) (Synonyms: rGHRH(1-29)NH2) 纯度: 98.22%

GRF (1-29) amide (rat) 是能刺激生长激素 (GH) 分泌的合成肽。

GRF (1-29) amide (rat)(Synonyms: rGHRH(1-29)NH2)

GRF (1-29) amide (rat) Chemical Structure

CAS No. : 91826-20-9

规格 价格 是否有货 数量
1 mg ￥1100 In-stock
5 mg ￥2200 In-stock
10 mg ￥3500 In-stock
25 mg ￥7500 In-stock
50 mg   询价  
100 mg   询价  

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GRF (1-29) amide (rat) 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GRF (1-29) amide (rat) is a synthetic peptide which can stimulate the growth hormone (GH) secretion.

体内研究
(In Vivo)

Time course studies of GRF (1-29) amide (rat) disappearance show apparent half-lives of 18±4 min and 13±3 min in serum and liver homogenate, respectively. This is accompanied by the appearance of degradation products that are all less hydrophobic than the native peptide. In the serum, two major metabolites are detected and isolated by preparative HPLC[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3473.08

Formula

C155H251N49O40S
CAS 号

91826-20-9
Sequence

His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-NH2
Sequence Shortening

HADAIFTSSYRRILGQLYARKLLHEIMNR-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

H2O : 16.67 mg/mL (4.80 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2879 mL 1.4396 mL 2.8793 mL
5 mM — — —
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Boulanger L, et al. Catabolism of rat growth hormone-releasing factor(1-29) amide in rat serum and liver. Peptides. 1992 Jul-Aug;13(4):681-9.
Cell Assay
[1]

To isolate GRF metabolites in the liver, rGRF(1-29)NH2 (10 mg) is first preincubated in 232 mL of Krebs’ buffer (5 min, 37°C) to help GRF solubilization and then incubated with a liver homogenate in a shaking bath at 37°C. The homogenate is prepared as in the degradation assays with 580 mg of liver (10 mg/mL). The reaction is stopped after 30 min by adding 174 mL of cold 50 mM phosphate solution (pH 0.8) and centrifugation (48,000×g, 20 min, 4°C). The supernatant is filtered twice and its pH is adjusted (3.0) with 6 N NaOH before chromatography. The GRF metabolites and residual rGRF(1-29)NH2 are isolated[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Boulanger L, et al. Catabolism of rat growth hormone-releasing factor(1-29) amide in rat serum and liver. Peptides. 1992 Jul-Aug;13(4):681-9.

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