NMS-859 | whatman (沃特曼)

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

NMS-859 纯度: 98.01%

NMS-859 是一种有效的，共价的 VCP (p97) 抑制剂，细胞中分别在 60 μM 和 1 mM ATP 的条件下，对野生型 VCP 的 IC₅₀ 值分别为 0.37 和 0.36 μM。

NMS-859 Chemical Structure

CAS No. : 1449236-96-7

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥4488	In-stock
2 mg	￥2333	In-stock
5 mg	￥3500	In-stock
10 mg	￥5000	In-stock
50 mg	￥15000	In-stock
100 mg	￥21000	In-stock
200 mg		询价
500 mg		询价

* Please select Quantity before adding items.

NMS-859 相关产品

•相关化合物库:

Covalent Screening Library Plus
Bioactive Compound Library Plus
Cell Cycle/DNA Damage Compound Library
Anti-Cancer Compound Library
Antiviral Compound Library
Anti-Aging Compound Library
Covalent Screening Library
Ubiquitination Compound Library
Endoplasmic Reticulum Stress Compound Library

生物活性

NMS-859 is a potent, covalent VCP (p97) inhibitor, with IC₅₀s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively.

IC₅₀ & Target

IC50: 360 nM (Cellular p97, 1 mM ATP), 370 nM (Cellular p97, 60 μM ATP)^[1]

体外研究
(In Vitro)

NMS-859 is a potent VCP inhibitor, with IC₅₀s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively. NMS-859 shows very weak inhibitory activity against VCP^C522T. NMS-859 also suppresses the proliferation of cells, with IC₅₀s of 3.5 μM and 3.0 μM in HCT116 and HeLa cell lines, respectively^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

349.79

Formula

C₁₅H₁₂ClN₃O₃S

CAS 号

1449236-96-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : ≥ 42 mg/mL (120.07 mM)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8589 mL	14.2943 mL	28.5886 mL
5 mM	0.5718 mL	2.8589 mL	5.7177 mL
10 mM	0.2859 mL	1.4294 mL	2.8589 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

Cell Assay ^[1]	Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with NMS-859 (eight dilution points, in duplicate) and incubated for an additional 72 h at 37°C under a 5% CO₂ atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase-based assay as a measure of cell viability. IC₅₀ values are calculated using the percentage of growth of treated cells versus the untreated control^[1]. 上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

Cell Assay
^[1]

Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with NMS-859 (eight dilution points, in duplicate) and incubated for an additional 72 h at 37°C under a 5% CO₂ atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase-based assay as a measure of cell viability. IC₅₀ values are calculated using the percentage of growth of treated cells versus the untreated control^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

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