Grape seed extract(Synonyms: 葡萄籽提取物)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Grape seed extract (Synonyms: 葡萄籽提取物)

Grape seed extract 是一种天然产物，具有抗炎和抗增殖作用。Grape seed extract 对脂肪代谢酶、胰脂肪酶和脂蛋白脂肪酶有抑制作用。Grape seed extract 能诱导人类结肠直肠癌细胞凋亡 (apoptotic)。

Grape seed extract(Synonyms: 葡萄籽提取物)

Grape seed extract Chemical Structure

CAS No. : 84929-27-1

规格 价格 是否有货 数量
100 mg ￥500 In-stock
250 mg ￥1000 In-stock
500 mg ￥1700 In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

Grape seed extract 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Drug Repurposing Compound Library Plus
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus

生物活性

Grape seed extract is a natural product, with anti-inflammatory and anti-proliferative effects. Grape seed extract shows inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase. Grape seed extract induces apoptotic in human colorectal cancer cells[1][2][3].

体外研究
(In Vitro)

Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells[3].
Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells[3].
Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 12 hours, 24 hours, 48 hours
Result: Inhibited cell growth in a dose- and a time-dependent manner.

Cell Cycle Analysis[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 12 hours, 24 hours, 48 hours
Result: Induced cell cycle arrest (a marked increase in Cip1/p21protein level and a decrease in G 1 phase^associated cyclins and cyclin-dependentkinases).

Western Blot Analysis[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 12 hours, 24 hours
Result: Modulates the protein levels of CDKIs, CDKs, and cyclins.

Apoptosis Analysis[3]

Cell Line: HT29 cells, LoVo cells
Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL
Incubation Time: 24 hours
Result: Showed a significant dose-dependent increase in apoptotic cell population in HT29 cells at 50 μg/mL and 100 μg/mL; all three Concentrations caused a dose-dependent increase in apoptotic cells.

体内研究
(In Vivo)

Grape seed extract (200 mg/kg; i.g.; 5 days/wk; for 8 weeks) inhibits HT29 colon carcinoma tumor xenograft growth in athymic nude mice[3].
Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors[3].
Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic male nu/nu mice, with human colon carcinoma HT29 tumor xenograft[3]
Dosage: 200 mg/kg
Administration: Oral gavage, 5 days/wk, for 8 weeks
Result: Showed time-dependent inhibition of tumor growth.

Clinical Trial

CAS 号

84929-27-1
中文名称

葡萄籽提取物； 葡萄籽精华； 葡萄籽萃取； 葡萄籽萃取物
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro: 

DMSO : 6 mg/mL (Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.6 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 0.6 mg/mL (Infinity mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 6.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.6 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 0.6 mg/mL (Infinity mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 6.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. The effects of grape seed extract (Vitis vinifera) supplement on inflammatory markers, neuropeptide Y, anthropometric measures, and appetite in obese or overweight individuals: A randomized clinical trial.

    [2]. Inhibitory effects of grape seed extract on lipases. Nutrition. 2003 Oct;19(10):876-9.

    [3]. Manjinder Kaur, et al. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells. Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):6194-202.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务