HBC514

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HBC514  纯度: ≥98.0%

HBC514 本身是一种非荧光的 HBC 模拟物，但在与 Pepper RNA 适配体形成紧密复合物后可以发出强烈的绿色荧光，用于 RNA 标记。在活的 Pepper 标记的细胞中，HBC514-Pepper complex 可以使 RNAs 可视化，并且可以通过简单的洗涤和染色灵活地改变荧光。

HBC514

HBC514 Chemical Structure

CAS No. : 2530161-67-0

规格 价格 是否有货 数量
5 mg ￥3500 In-stock
10 mg ￥6350 In-stock
25 mg ￥11500 In-stock
50 mg ￥18500 In-stock
100 mg   询价  
200 mg   询价  

* Please select Quantity before adding items.

HBC514 相关产品

•相关化合物库:

  • Covalent Screening Library Plus
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  • Anti-Cancer Compound Library
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  • Covalent Screening Library

生物活性

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1].

IC50 & Target

IC50: RNA fluorescent dye
体外研究
(In Vitro)

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells[1].
‘Pepper’ has reduced Mg2+ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness[1].
HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9)[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

330.43

Formula

C21H22N4
CAS 号

2530161-67-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 25 mg/mL (75.66 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.0264 mL 15.1318 mL 30.2636 mL
5 mM 0.6053 mL 3.0264 mL 6.0527 mL
10 mM 0.3026 mL 1.5132 mL 3.0264 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.Nat Biotechnol. 2019 Nov;37(11):1287-1293.

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