Hematein

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Hematein  纯度: 74.90%

Hematein 是苏木精的氧化产物，可作为染料。Hematein 是一种变构酪蛋白激酶 II 抑制剂，IC50 为 0.74 μM。Hematein 抑制 Akt/PKB Ser129 磷酸化，Wnt/TCF 途径并增加肺癌细胞的凋亡。

Hematein

Hematein Chemical Structure

CAS No. : 475-25-2

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥610 In-stock
500 mg ￥550 In-stock
1 g ￥990 In-stock
5 g   询价  
10 g   询价  

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生物活性

Hematein is a oxidation product of hematoxylin acted as a dye[1]. Hematein is an allosteric casein kinase II inhibitor with an IC50 of 0.74 μM. Hematein inhibits Akt/PKB Ser129 phosphorylation, the Wnt/TCF pathway and increases apoptosis in lung cancer cells[2].

IC50 & Target[2]

CKII

0.74 μM (IC50)

Akt

 

Wnt

 

体外研究
(In Vitro)

Hematein (10-100 μM; 14 days) inhibits cells growth in A427 lung cancer cells[1].
Hematein (50 and 100 μM; 48 hours) induces apoptosis in A427 lung cancer cells[1].
Hematein (50 and 100 μM; 48 hours) inhibits CK2-specific Akt phosphorylation[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A427 (HTB-53) cell line
Concentration: 10-100 μM
Incubation Time: 14 days
Result: Inhibited cells growth.

Apoptosis Analysis[1]

Cell Line: A427 (HTB-53) cell line
Concentration: 50 and 100 μM
Incubation Time: 48 hours
Result: Induced apoptosis in A427 lung cancer cells.

Western Blot Analysis[1]

Cell Line: A427 (HTB-53) cell line
Concentration: 50 and 100 μM
Incubation Time: 48 hours
Result: Inhibited CK2-specific Akt phosphorylation.

体内研究
(In Vivo)

Hematein (intraperitoneal injections; 50 mg/kg; twice a week for 6 weeks) inhibits tumor growth in A427 lung cancer cell xenografts[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female athymic BALB/c nude mice (6-week-old) [1]
Dosage: 50 mg/kg
Administration: Intraperitoneal injections; twice a week for 6 weeks
Result: Inhibited tumor growth in A427 lung cancer cell xenografts.

分子量

300.26

Formula

C16H12O6
CAS 号

475-25-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (333.04 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3304 mL 16.6522 mL 33.3045 mL
5 mM 0.6661 mL 3.3304 mL 6.6609 mL
10 mM 0.3330 mL 1.6652 mL 3.3304 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.33 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Shirai, K., & Matsuoka, M. (1996). Structure and properties of hematein derivatives. Dyes and Pigments, 32(3), 159–169.

    [2]. Hung MS, et al. Hematein, a casein kinase II inhibitor, inhibits lung cancer tumor growth in a murine xenograftmodel.Int J Oncol. 2013 Nov;43(5):1517-22.

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