Sparstolonin B

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Sparstolonin B  纯度: 99.50%

Sparstolonin B 作为选择性 TLR2 和 TLR4 拮抗剂，选择性阻断 TLR2 和 TLR4 介导的炎症信号传导。Sparstolonin B 具有抗 HIV 和抗癌活性。

Sparstolonin B

Sparstolonin B Chemical Structure

CAS No. : 1259330-61-4

规格 价格 是否有货 数量
5 mg ￥2800 In-stock
10 mg ￥4500 询价
50 mg   询价  
100 mg   询价  

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生物活性

Sparstolonin B acts as a selective TLR2 and TLR4 antagonist and selectively blocks TLR2- and TLR4-mediated inflammatory signaling. Sparstolonin B has anti-HIV and anticancer activities[1][2].

IC50 & Target[1][2]

TLR2

 

TLR4

 

HIV-1

 

体外研究
(In Vitro)

Sparstolonin B (1-20 µM; 2-4 days) inhibits cell growth and viability of neuroblastoma cells[3].
Sparstolonin B inhibits TLR ligand-induced cytokine expression in mouse macrophages. Sparstolonin B inhibits MyD88 recruitment to TLR4 and TLR2[1].
Sparstolonin B generates reactive oxygen species (ROS) in neuroblastoma cells. Sparstolonin B reduces expression of N-myc in neuroblastoma cells[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: SH-SY5Y, IMR-32, NGP, SKNF-1 and SK-N-BE(2) cells
Concentration: 1 µM, 5 µM, 10 µM or 20 µM
Incubation Time: 2-4 days
Result: Effectively and dose-dependently inhibits the viability of all neuroblastoma cell lines after 2 days (SH-SY5Y and IMR-32), 3 days (NGP cells) or 4 days (SKNF-1 and SK-N-BE(2) cells) treatment.

体内研究
(In Vivo)

Sparstolonin B (100 μg/mouse; i.p.) suppresses LPS-provoked inflammation in mice[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-6-week-old male C57Bl/6 mice (body weight 18-20 g)[1]
Dosage: 100 μg/mouse
Administration: I.p.
Result: Significantly lower TNFα and IL-1β expression levels in LPS-induced sepsis mouse model.

分子量

268.22

Formula

C15H8O5
CAS 号

1259330-61-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 10 mg/mL (37.28 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.7283 mL 18.6414 mL 37.2828 mL
5 mM 0.7457 mL 3.7283 mL 7.4566 mL
10 mM 0.3728 mL 1.8641 mL 3.7283 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Liang Q, et al. Characterization of sparstolonin B, a Chinese herb-derived compound, as a selective Toll-like receptor antagonist with potent anti-inflammatory properties. J Biol Chem. 2011;286(30):26470-26479.

    [2]. Deng X, et al. The Chinese herb-derived Sparstolonin B suppresses HIV-1 transcription. Virol J. 2015;12:108. Published 2015 Jul 25.

    [3]. Kumar A, et al. Sparstolonin B, a novel plant derived compound, arrests cell cycle and induces apoptosis in N-myc amplified and N-myc nonamplified neuroblastoma cells [published correction appears in PLoS One. 2016;11(7):e0159082]. PLoS One. 2014;9(5):e96343. Published 2014 May 1.

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