QX77

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QX77  纯度: 99.48%

QX77 是分子伴侣介导的自噬 (CMA) 激活剂，可上调 LAMP2A 的表达。QX77 诱导 Rab11 上调，挽救 Rab11 下调和转运缺陷。QX77 可抑制 ES 细胞自我更新，促进分化。

QX77

QX77 Chemical Structure

CAS No. : 1798331-92-6

规格 价格 是否有货 数量
5 mg ￥900 In-stock
10 mg ￥1500 In-stock
50 mg ￥4500 In-stock
100 mg ￥8500 In-stock
200 mg   询价  
500 mg   询价  

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QX77 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus

生物活性

QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells[1]. QX77 can impede self-renewal and promote differentiation of ES cells[2].

IC50 & Target

CMA[1]
体外研究
(In Vitro)

QX77 (48 hours) upregulates Rab11 expression levels in Ctns-/- MEFs[1].
In Ctns-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells[1].
Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane[1].
QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells[1].
QX77 protects cystinotic cells from the increased susceptibility to tert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression[1].
QX77 (10 μM; 0, 3 or 6 days) activates CMA and increases LAMP2A expression in D3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: ES D3 cell line; E14TG2a (E14) cell line
Concentration: 10 μM
Incubation Time: 3 or 6 days
Result: Increased LAMP2A expression and decreased SOX2 and Oct4 protein expression.

分子量

300.74

Formula

C16H13ClN2O2
CAS 号

1798331-92-6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : < 1 mg/mL (ultrasonic) (insoluble or slightly soluble)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 50% PEG300    50% saline

    Solubility: 10 mg/mL (33.25 mM); Suspended solution; Need ultrasonic

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Jinzhong Zhang, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017 Jun 23;292(25):10328-10346

    [2]. Yi Xu, et al. Chaperone-mediated autophagy regulates the pluripotency of embryonic stem cells. Science. 2020 Jul 24;369(6502):397-403.

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