LOM612 is a potent FOXO relocator, with an EC₅₀ value of 1.5 μM in U2fox RELOC cells^[1].

EC50: 1.5 μM (FOXO, in U2fox RELOC cells)^[1]

LOM612 potently activates nuclear translocation of FOXO with an EC₅₀ value of 1.5 μM, and this effect is independent of CRM-1. LOM612 effectively induces translocation of endogenous FOXO3a and FOXO1, and increases the expression of the FOXO target genes p27 and FasL. LOM612 shows no effect on the nuclear export of endogenous NFKB2 transcription factor in U2OS cells. LOM612 is cytotoxic to HepG2 cells, with an IC₅₀ value of 0.64 μM, and does not sensitize non-cancer THLE2 cells (IC₅₀, 2.76 μM)^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

258.30

C₁₃H₁₀N₂O₂S

2173232-79-4

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

In Vitro: 

DMSO : 6 mg/mL (23.23 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. Cautain B, et al. Discovery of a Novel, Isothiazolonaphthoquinone-Based Small Molecule Activator of FOXO Nuclear-Cytoplasmic Shuttling. PLoS One. 2016 Dec 9;11(12):e0167491.

Cells are seeded at a concentration of 1× 10⁴ cells/well in 200 μL culture medium and incubated at 37°C in 5% CO₂. After 24 hours, when the monolayer formed, the medium is replaced with a final volume of 200 μL of new medium with tested compounds (LOM612, etc.) or controls are added to the plates. Cells are treated with eight 2-fold serial dilutions of each compound spanning concentrations from 50 μM to 0.39 μM in 1% DMSO final. Controls are on the first and the last columns of the plates. On the first column, methyl methanesulfonate (MMS) acts as a positive control and DMSO as a negative control. When compounds (LOM612, etc.) and controls are added, plates are incubated at 37°C in 5% CO₂ incubator for 72 hours. After this time, MTT solution is prepared at 5 mg/mL in PBS 1X and then diluted at 0.5 mg/mL in MEM without phenol red. The sample solution in wells is flicked off and 100 μL of MTT dye is added to each well. The plates are gently shaken and incubated for 3 hours at 37°C in 5% CO₂ incubator. The supernatant is removed and 100 μL of DMSO 100% is added. The plates are gently shaken to solubilize the formed formazan. The absorbance is measured at a wavelength of 570 nm^[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Cautain B, et al. Discovery of a Novel, Isothiazolonaphthoquinone-Based Small Molecule Activator of FOXO Nuclear-Cytoplasmic Shuttling. PLoS One. 2016 Dec 9;11(12):e0167491.