Costunolide(Synonyms: 木香烃内酯; (+)-Costunolide; Costus lactone)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Costunolide (Synonyms: 木香烃内酯; (+)-Costunolide; Costus lactone) 纯度: 99.97%

Costunolide ((+)-Costunolide) 是一种天然的倍半萜内酯，它具有抗氧化、抗炎、抗过敏、骨重塑、神经保护、促进毛发生长、抗癌和抗糖尿病的特性。Costunolide 可诱导乳腺癌细胞周期阻滞和凋亡 (apoptosis)。

Costunolide(Synonyms: 木香烃内酯; (+)-Costunolide; Costus lactone)

Costunolide Chemical Structure

CAS No. : 553-21-9

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ￥614 In-stock
5 mg ￥558 In-stock
10 mg ￥837 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Costunolide 相关产品

•相关化合物库:

  • Covalent Screening Library Plus
  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Human Endogenous Metabolite Compound Library
  • Covalent Screening Library
  • Diabetes Related Compound Library
  • Terpenoids Library
  • Traditional Chinese Medicine Monomer Library
  • FDA Approved & Pharmacopeial Drug Library
  • Neuroprotective Compound Library
  • Anti-Breast Cancer Compound Library
  • Food-Sourced Compound Library

生物活性

Costunolide ((+)-Costunolide) is a naturally occurring sesquiterpene lactone, with antioxidative, anti-inflammatory, antiallergic, bone remodeling, neuroprotective, hair growth promoting, anticancer, and antidiabetic properties. Costunolide can induce cell cycle arrest and apoptosis on breast cancer cells[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Costunolide inhibits the colony formation, migrative and invasive abilities of the H1299 cells in a dose or time dependent manner[2].
Costunolide (6.7-215.2 μM; 24 hours) inhibits the viability of H1299 cells in a dose-dependent manner, with an IC50 of 23.93 µM[2].
Costunolide (12.0-48.0 µM; 48 hours) induces apoptosis in H1299 cells[2].
Costunolide (12-48.0 µM; 6-12 hours) regulates metastasis- and proliferation-associated mRNA expression[2].
Costunolide regulates epithelial-to-mesenchymal transition (EMT)-associated protein expression[2].
Costunolide regulates c-Myc mediated apoptosis signaling and 14-3-3-mediated signaling pathways in breast cancer cells[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: H1299 cells
Concentration: 6.7 μM, 13.5 μM, 26.9 μM, 107.6 μM, 215.2 μM
Incubation Time: 24 hours
Result: Inhibited the viability of H1299 cells (MTT assay).

Apoptosis Analysis[2]

Cell Line: H1299 cells
Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
Incubation Time: 48 hours
Result: Significantly promoted apoptosis at 24.0 µM and 48.0 µM.

RT-PCR[2]

Cell Line: H1299 cells
Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
Incubation Time: 6 hours, 12 hours
Result: Regulated the metastasis- and proliferation-associated mRNA levels in a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: H1299 cells
Concentration: 0 µM, 12.0 µM, 24.0 µM, 48.0 µM
Incubation Time: 48 hours
Result: Significantly inhibited the EMT of H1299 cells.

体内研究
(In Vivo)

Costunolide (20 mg/kg; i.p; daily; for 30 days) inhibits breast cancer through c-Myc/p53 and AKT/14-3-3 pathway[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 4 weeks old female BALB/c nude mice, MDA-MB-231 cells xenograft mouse models[3]
Dosage: 20 mg/kg
Administration: Intraperitoneal injection, daily, for 30 days
Result: Reduced the expression levels of c-Myc and p-AKT and elevated the expression levels of p53 and p-14-3-3.

分子量

232.32

Formula

C15H20O2
CAS 号

553-21-9
中文名称

木香烃内酯；木烃香内酯
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro: 

DMSO : ≥ 49 mg/mL (210.92 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.3044 mL 21.5220 mL 43.0441 mL
5 mM 0.8609 mL 4.3044 mL 8.6088 mL
10 mM 0.4304 mL 2.1522 mL 4.3044 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (8.95 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (8.95 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (8.95 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Dae Yong Kim, et al. Costunolide-A Bioactive Sesquiterpene Lactone with Diverse Therapeutic Potential. Int J Mol Sci. 2019 Jun; 20(12): 2926.

    [2]. Minyan Wei, et al. Costunolide induces apoptosis and inhibits migration and invasion in H1299 lung cancer cells. Oncol Rep. 2020 Jun;43(6):1986-1994.

    [3]. Zhangxiao Peng, et al. Costunolide and dehydrocostuslactone combination treatment inhibit breast cancer by inducing cell cycle arrest and apoptosis through c-Myc/p53 and AKT/14-3-3 pathway.Sci Rep. 2017; 7: 41254.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务