NNK

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

NNK 

NNK 是一种尼古丁亚硝化衍生物。NNK 通过激活 ERK1/2 和 PKCα， 同时在 Ser70 刺激 Bcl2 磷酸化，以及在Thr58 和 Ser62 刺激 c-Myc。NNK 能诱导人肺癌细胞的存活和增殖。NNK 可用于构建肺癌小鼠模型。

NNK

NNK Chemical Structure

CAS No. : 64091-91-4

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生物活性

NNK is a nicotine-nitrosated derivative. NNK simultaneously stimulates Bcl2 phosphorylation exclusively at Ser70 and c-Myc at Thr58 and Ser62 through activation of both ERK1/2 and PKCα[1]. NNK induces survival and proliferation of human lung cancer cells. NNK can be used for lung cancer mice model structure[2].

IC50 & Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

NNK (100 pM; 0-60 min) stimulates activation of PKCα and MAPKs ERK1/2 that directly induce c-Myc phosphorylation[1].
NNK (100 pM; 96 hours) enhances proliferation of cells expressing WT but not AA c-Myc mutant[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line: NCI-H82 cells[1]
Concentration: 100 pM
Incubation Time: 0-60 min
Result: Stimulated activation of PKCα and MAPKs ERK1/2 that directly induced c-Myc phosphorylation.

Apoptosis Analysis

Cell Line: H1299 lung cancer cells[1]
Concentration: 100 pM
Incubation Time: 96 hours
Result: Enhanced proliferation of cells expressing WT but not T58A/S62A c-Myc mutant.

分子量

207.23

Formula

C10H13N3O2
CAS 号

64091-91-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jin Z, Gao F, Flagg T, Deng X. Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone promotes functional cooperation of Bcl2 and c-Myc through phosphorylation in regulating cell survival and proliferation. J Biol Chem. 2004;279(38):40209-40219.

    [2]. Castonguay A, Pepin P, Stoner GD. Lung tumorigenicity of NNK given orally to A/J mice: its application to chemopreventive efficacy studies. Exp Lung Res. 1991;17(2):485-499.

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