Withanolide B

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Withanolide B 

Withanolide B 是 W. somnifera Dunal 的活性成分。Withanolide B 通过 ERK1/2 和 Wnt/β-catenin 信号通路促进 hBMSCs 的成骨分化。 Withanolide B 具有神经保护、抗关节炎、抗衰老和抗癌的作用。

Withanolide B

Withanolide B Chemical Structure

CAS No. : 56973-41-2

规格 是否有货
5 mg 询价
10 mg 询价
25 mg 询价

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生物活性

Withanolide B is an active component of W. somnifera Dunal. Withanolide B promotes osteogenic differentiation of hBMSCs via ERK1/2 and Wnt/β-catenin signaling pathways. Withanolide B exhibits neuroprotective, anti-arthritic, anti-aging and anti-cancer effects[1][2][3].

体外研究
(In Vitro)

Withanolide B (1-100 nM; 3-5 days) significantly increases the expression of COL1A1 and RUNX2 genes and proteins in hBMSCs[1].
Withanolide B (1-100 nM; 11 or 3 days) increases the formation of extracellular matrix calcium deposits and increased the activity of alkaline phosphatase (ALP)[1].
Withanolide B (1-100 nM; 3-5 days) increases the protein expressions of p-ERK and active β-catenin of hBMSCs[1].
Withanolide B (10-100 μM; 48 h) reverses the Aβ42 aggregation-induced toxicy in SK-N-SH cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Human bone mesenchymal stem cells
Concentration: 1, 10, 100 nM
Incubation Time: 3, 5 days
Result: Significantly increased the expression of COL1A1 and RUNX2.

体内研究
(In Vivo)

Withanolide B (10 mg/kg; topical administration) promotes bone healing in a rat tibial defect model[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (8 weeks, 200 g) with tibial defect[1]
Dosage: 10 mg/kg
Administration: Injected in situ at the bone defect site at different time points (0, 3, 5, 7, and 9 days)
Result: Increased the trabecular number, trabecular thickness and the thickness of the cortical bone.

分子量

454.60

Formula

C28H38O5
CAS 号

56973-41-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kuang Z, et, al. Withanolide B promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via ERK1/2 and Wnt/β-catenin signaling pathways. Int Immunopharmacol. 2020 Nov;88:106960.

    [2]. Dubey S, et, al. Improving the inhibition of β-amyloid aggregation by withanolide and withanoside derivatives. Int J Biol Macromol. 2021 Mar 15;173:56-65.

    [3]. Sivanandha G, et, al. Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor. PLoS One. 2014 Aug 4;9(8):e104005.

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