Nemadipine-A

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Nemadipine-A 

Nemadipine-A 是 EGL-19 Ca2+ 通道 (Ca2+ channel) 的特异性抑制剂。Nemadipine-A 是一种细胞渗透性 L 型钙通道抑制剂，可使对 TRAIL 抗性的癌细胞对该配体敏感。

Nemadipine-A

Nemadipine-A Chemical Structure

CAS No. : 54280-71-6

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生物活性

Nemadipine-A is a specific inhibitor of the EGL-19 L-type Ca2+ channel[1]. Nemadipine-A, a cell-permeable L-type calcium channel inhibitor, sensitizes TRAIL-resistant cancer cells to this ligand[2].

体外研究
(In Vitro)

Nemadipine-A is a specific and highly effective inhibitor of EGL-19. Nemadipine-A (µM) is sufficient to abolish the enhanced level of paralysis observed in egl-19(ad695); crIs4 mutants[1].
Nemadipine-A strongly potentiates TRAIL-induced apoptosis in TRAIL-resistant lung cancer cells via the down-regulation of the anti-apoptotic protein survivin[2]. Nemadipine-A sensitizes H1299 lung cancer cells towards TRAIL-induced cytotoxicity[2]. Combination of Nemadipine-A (20 μM) with TRAIL (20 ng/mL) induces caspase-mediated apoptotic cell death[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: H1299 lung adenocarcinoma cells
Concentration: 0, 5, 10, 20, and 30 μM
Incubation Time: 8 hours
Result: H1299 cells were resistant to the apoptotic effects of TRAIL, but the co-administration of Nemadipine-A and TRAIL in these cells resulted in notably increased cytotoxicity in a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: H1299 cells
Concentration: 20 μM
Incubation Time: 8 hours
Result: Co-treated with TRAIL and Nemadipine-A (T/N; 20 ng/mL and 20 μM respectively) induced cell death in H1299 cells.

分子量

419.34

Formula

C19H18F5NO4
CAS 号

54280-71-6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Peter I Joyce, et al. The atypical calpains: evolutionary analyses and roles in Caenorhabditis elegans cellular. PLoS Genet. 2012;8(3):e1002602.degeneration

    [2]. Seong Ho Park, et al. Down-Regulation of Survivin by Nemadipine-A Sensitizes Cancer Cells to TRAIL-Induced Apoptosis. Biomol Ther (Seoul). 2013 Jan;21(1):29-34.

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