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8-Aminoadenosine (Synonyms: 8-氨基腺苷; 8-NH2-Ado)
8-Aminoadenosine (8-NH2-Ado) 是一种 RNA 导向的核苷类似物，可降低细胞 ATP 水平并抑制 mRNA 合成。8-Aminoadenosine 阻断 Akt/mTOR 信号，并诱导 p53 非依赖性的自噬和细胞凋亡。8-Aminoadenosine 具有抗肿瘤活性。
8-Aminoadenosine Chemical Structure
CAS No. : 3868-33-5
| 规格 |
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是否有货 |
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| 100 mg |
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询价 |
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| 250 mg |
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询价 |
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| 500 mg |
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询价 |
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* Please select Quantity before adding items.
| 生物活性 |
8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibits mRNA synthesis. 8-Aminoadenosine blocks Akt/mTOR signaling and induces autophagy and apoptosis in a p53-independent manner. 8-Aminoadenosine has antitumor activity[1][2][3].
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| IC50 & Target[1][2] |
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体外研究
(In Vitro) |
8-Aminoadenosine (8-NH2-Ado; 0.1-10 μM; for 48 h) has IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively[1].
8-Aminoadenosine (10 μM; for 24 h) induces significant apoptotic death of MCF-7 cells in p53-independent pathway. 8-Aminoadenosine causes PARP cleavage in MCF-7 cells[2].
8-Aminoadenosine (3 μM; 0.5-4 h) induces autophagy in the MM.1S cell line[1].
8-Aminoadenosine (3 μM; 2-16 h) causes a greater drop in ATP levels in the MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) causes a 50% reduction in glucose consumption in MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) indicates a time-dependent decrease in GLUT1 expression at 5 h, whereas at 24 h there was a down-regulation of both transporters (GLUT1 and GLUT4) in MM.1S cells[1].
8-Aminoadenosine inhibits cell proliferation, activated cell death, and does not activate transcription of the p53 target gene p21 or increase protein levels of either p53 or p21[1].
The toxic effects of 8-Aminoadenosine require adenosine kinase activity to convert 8-Aminoadenosine to 8-NH2-ATP in adenosine kinase-deficient cells[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
| Cell Line: |
MM.1S and U266 cells |
| Concentration: |
0.1, 0.3, 1, 3, 10 μM |
| Incubation Time: |
For 48 hours |
| Result: |
Had IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively. |
Apoptosis Analysis[2]
| Cell Line: |
MCF-7 cells |
| Concentration: |
10 μM |
| Incubation Time: |
For 24 hours |
| Result: |
Induced significant apoptotic death.
Apoptosis was not inhibited by knockdown of functional p53. |
Apoptosis Analysis[1]
| Cell Line: |
MM.1S cell line |
| Concentration: |
3 μM |
| Incubation Time: |
0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 hours |
| Result: |
Induced the formation of LC3-II protein.
Caused the appearance of a population with a high AVO content with 1 μM for 24 hours. |
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| 中文名称 |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 溶解性数据 |
In Vitro:
DMSO : 83.33 mg/mL (295.22 mM; Need ultrasonic)
配制储备液
| 浓度 溶剂体积 质量 |
1 mg |
5 mg |
10 mg |
| 1 mM |
3.5428 mL |
17.7142 mL |
35.4283 mL |
| 5 mM |
0.7086 mL |
3.5428 mL |
7.0857 mL |
| 10 mM |
0.3543 mL |
1.7714 mL |
3.5428 mL |
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| 参考文献 |
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[1]. Mala Shanmugam, et al. Targeting glucose consumption and autophagy in myeloma with the novel nucleoside analogue 8-aminoadenosine. J Biol Chem. 2009 Sep 25;284(39):26816-30.
[2]. Alla Polotskaia, et al. 8-Amino-adenosine activates p53-independent cell death of metastatic breast cancers. Mol Cancer Ther. 2012 Nov;11(11):2495-504.
[3]. Jennifer Ann Frey, et al. 8-Amino-adenosine inhibits multiple mechanisms of transcription. Mol Cancer Ther. 2010 Jan;9(1):236-45.
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