Phoyunnanin E, isolated from Dendrobium venustum, possesses anti-migration activity. Phoyunnanin E can be used for the research of cancer[1].
体外研究 (In Vitro)
Phoyunnanin E (0~10 μM; 48 hours; H460 cells) decreases cell migration through integrin downregulation[1]. Phoyunnanin E (0~100 μM; 24 and 48 hours; H460 cells) shows that the proliferation rate of treated cells have significantly decreased in a dose-dependent manner at the concentration of 10 μM at 48 hours[1]. Phoyunnanin E (0~100 μM; 24 hours; H460 cells) induces cytotoxic effects at the concentrations from 50 to 100 μM and induces a significant increase in apoptotic and necrotic cells at 20~100 μM[1]. Phoyunnanin E (0~10 μM; 48 hours; H460, H292 and A549 cells) significantly decreases the number of cells invading across the matrix and transwell filter within 24 hours in a dose-dependent manner[1]. Phoyunnanin E attenuates anchorage-independent growth and migration of lung cancer H460 cells. Phoyunnanin E significantly inhibits cell migration across the wound space at the concentrations of 5 and 10 μM, at 24 h and 48 h, compared to the non-treated control. Phoyunnanin E (0~10 μM; 48 hours) exhibits a significant decrease in filopodia numbers at the protrusion edges of the cells in a dose-dependent manner. Phoyunnanin E decreases cancer cell migration in other human lung cancer cells. Phoyunnanin E decreases cancer cell invasion. Phoyunnanin E suppresses epithelial-mesenchymal transition (EMT). Phoyunnanin E down-regulates integrins αv and β3 and the regulatory proteins in cell migration[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis[1]
Cell Line:
H460 cells
Concentration:
1~10 μM
Incubation Time:
48 hours
Result:
Decreased cell migration through integrin downregulation.
Cell Proliferation Assay[1]
Cell Line:
H460 cells
Concentration:
0~100 μM
Incubation Time:
24 and 48 hours
Result:
The proliferation rate of treated cells had significantly decreased in a dose-dependent manner at the concentration of 10 μM at 48 h.
Apoptosis Analysis[1]
Cell Line:
H460 cells
Concentration:
0~100 μM
Incubation Time:
24 hours
Result:
Induced a significant increase in apoptotic and necrotic cells at 20~100 μM.
Cell Cytotoxicity Assay[1]
Cell Line:
H460 cells
Concentration:
0~100 μM
Incubation Time:
24 hours
Result:
Induced cytotoxic effects at the concentrations from 50 to 100 μM.
Immunofluorescence[1]
Cell Line:
H460, H292 and A549 cells
Concentration:
0~10 μM
Incubation Time:
48 hours
Result:
Significantly decreased the number of cells invading across the matrix and transwell filter within 24 hours in a dose-dependent manner.
分子量
482.52
Formula
C30H26O6
CAS 号
886747-60-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Petpiroon N, et al. Phoyunnanin E inhibits migration of non-small cell lung cancer cells via suppression of epithelial-to-mesenchymal transition and integrin αv and integrin β3. BMC Complement Altern Med. 2017;17(1):553. Published 2017 Dec 29.
