D-3

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

D-3 

D-3 是一种磷酸化肽，是一种高效、简单、特异的 IPSC 消除剂。

D-3

D-3 Chemical Structure

CAS No. : 1967815-98-0

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生物活性

D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent[1].

体外研究
(In Vitro)

D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay[1].
D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM[1].
D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment[1].
D-3 has little influence on various non-iPSCs, including hepatocytes and neurons[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Six human iPSCs lines and one human ESC line (khES1).
Concentration: 400 μM.
Incubation Time: 1-2 h.
Result: Sufficient to induce a viability loss (>99% in all iPSC lines and >95% in khES1).

Clinical Trial

分子量

870.88

Formula

C48H47N4O10P
CAS 号

1967815-98-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Yi Kuang, et al. Efficient, Selective Removal of Human Pluripotent Stem Cells via Ecto-Alkaline Phosphatase-Mediated Aggregation of Synthetic Peptides. Cell Chem Biol. 2017 Jun 22;24(6):685-694.e4.

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