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Phosphoramide mustard (Synonyms: 磷酰胺氮芥)
Phosphoramide mustard 是环磷酰胺 (HY-17420) 的活性代谢物，具有抗肿瘤活性。Phosphoramide mustard 能诱导 DNA 损伤。
Phosphoramide mustard Chemical Structure
CAS No. : 10159-53-2
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是否有货 |
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| 100 mg |
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询价 |
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| 250 mg |
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询价 |
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| 500 mg |
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* Please select Quantity before adding items.
Phosphoramide mustard 的其他形式现货产品:
Phosphoramide mustard (cyclohexanamine)
| 生物活性 |
Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage[1][2].
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IC50 & Target |
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体外研究
(In Vitro) |
Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1].
Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1].
Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1].
Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
| Cell Line: |
SIGCs |
| Concentration: |
0.5 μM, 1 μM, 3 μM, 6 μM |
| Incubation Time: |
48 hours |
| Result: |
Reduced cell viability at concentrations of 3 μM and higher. |
RT-PCR[1]
| Cell Line: |
SIGCs |
| Concentration: |
3 μM, 6 μM |
| Incubation Time: |
24 hours, 48 hours |
| Result: |
Increased DDR gene mRNA expression levels. |
Western Blot Analysis[1]
| Cell Line: |
SIGCs |
| Concentration: |
3 μM, 6 μM |
| Incubation Time: |
24 hours, 48 hours |
| Result: |
Generally increased DDR proteins. |
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体内研究
(In Vivo) |
Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats[2].
Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].
上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: |
Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2] |
| Dosage: |
2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg |
| Administration: |
Intraperitoneal injection, once daily, for 5 consecutive days |
| Result: |
Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg. |
| Animal Model: |
Rats[2] |
| Dosage: |
59.4 mg/kg (Pharmacokinetic Analysis) |
| Administration: |
Intravenous injection |
| Result: |
T1/2 (15.1 min). |
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| 中文名称 |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 参考文献 |
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[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258.
[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7.
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