Gp100 (25-33), mouse(Synonyms: Mgp100 (25-33))

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Gp100 (25-33), mouse (Synonyms: Mgp100 (25-33))

Gp100 (25-33), mouse 序列位于小鼠自身/肿瘤抗原糖蛋白 (Mgp100) 的第25-33个残基。Mgp100 (25-33), mouse是一种参与色素合成的酶，其表位片段在正常黑色素细胞和黑色素瘤细胞中均有表达。

Gp100 (25-33), mouse(Synonyms: Mgp100 (25-33))

Gp100 (25-33), mouse Chemical Structure

CAS No. : 212370-36-0

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Gp100 (25-33), mouse 的其他形式现货产品:

Gp100 (25-33), mouse TFA

生物活性

Gp100 (25-33), mouse sequence is found in residues 25 to 33 of the mouse self/tumor antigen glycoprotein (mgp100). Mgp100 is an enzyme involved in pigment synthesis, and the epitope fragment is expressed in both normal melanocytes and melanoma cells[1].

分子量

1104.13

Formula

C46H69N15O17
CAS 号

212370-36-0
Sequence

Glu-Gly-Ser-Arg-Asn-Gln-Asp-Trp-Leu
Sequence Shortening

EGSRNQDWL
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Gal Cafri, et al. Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. PLoS One. 2013;8(2):e55583.

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