Bryostatin 1(Synonyms: 苔藓抑素1)

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Bryostatin 1 (Synonyms: 苔藓抑素1) 纯度: ≥99.0%

Bryostatin 1 是一种天然的大环内酯类化合物，从苔藓虫 Bugula neritina 中分离出来，也是一种有效的中枢神经系统 (CNS) 渗透性的 PKC 调节剂。Bryostatin 1 以 Ki 值为 8.07 nM 和 0.45 nM 分别结合到分离的 Munc13-1 的 C1 域和全长的 Munc13-1 蛋白。Bryostatin 1 具有抗癌，抗炎，神经保护，抗 HIV-1 感染的特性。

Bryostatin 1(Synonyms: 苔藓抑素1)

Bryostatin 1 Chemical Structure

CAS No. : 83314-01-6

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10 μg ￥6200 In-stock

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生物活性

Bryostatin 1 is a natural macrolide isolated from the bryozoan Bugula neritina and is a potent and central nervous system (CNS)-permeable PKC modulator. Bryostatin 1 binds to the isolated C1 domain of Munc13-1 and the full-length Munc13-1 protein with Kis of 8.07 nM and 0.45 nM, respectively. Bryostatin 1 has anti-cancer, anti-inflammatory, neuroprotective, anti-HIV-1 infection properties[1][2][3][4].

IC50 & Target

PKC[1]
HIV-1[4]
体外研究
(In Vitro)

Bryostatin 1 (1 µM; 5 minutes; HT22 cells) treatment successfully recruits Munc13-1 from the cytosol to the plasma membrane. Effects of Bryostatin 1 on the other Munc13 family members, ubMunc13-2 and bMunc13-2, resembled those of Munc13-1 for translocation [1].
The increased level of expression of Munc13-1 following a 24 h incubation with Bryostatin 1 in both HT22 and primary mouse hippocampal cells is observed[1].
Bryostatin 1 can also affect the immune system by modulating dendritic cells (DCs) via toll-like receptor 4 (TLR4) through the MyD88-independent pathway, which favors an anti-inflammatory environment by inducing a type 2 phenotype that promotes the differentiation of CD4+ T-helper (Th) lymphocytes into Th2 versus Th1 effector cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HT22 cells
Concentration: 1 µM
Incubation Time: 5 minutes
Result: Caused Munc13-1 to transfer to the membrane fraction.

体内研究
(In Vivo)

Bryostatin 1 (30 μg/kg; intraperitoneal injection; 3 d per week; for 2 weeks; C57BL/6J mice) treatment abolishes the onset of EAE[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6J mice (8-12-week-old) with MOG35-55[2]
Dosage: 30 μg/kg
Administration: Intraperitoneal injection; 3 d per week; for 2 weeks
Result: Abolished the onset of experimental autoimmune encephalomyelitis (EAE).

Clinical Trial

分子量

905.03

Formula

C47H68O17
CAS 号

83314-01-6
中文名称

苔藓抑素1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
参考文献

  • [1]. Blanco FA, et al. Munc13 Is a Molecular Target of Bryostatin 1. Biochemistry. 2019 Jul 9;58(27):3016-3030.

    [2]. Kornberg MD, et al. Bryostatin-1 alleviates experimental multiple sclerosis. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2186-2191.

    [3]. Zeng N, et al. Bryostatin 1 causes attenuation of TPA-mediated tumor promotion in mouse skin. Mol Med Rep. 2018 Jan;17(1):1077-1082.

    [4]. Proust A, et al. HIV-1 infection and latency-reversing agents bryostatin-1 and JQ1 disrupt amyloid beta homeostasis in human astrocytes. Glia. 2020 Apr 6.

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