Autophagy inducer 4

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Autophagy inducer 4 

Autophagy inducer 4 是一种基于 Magnolol 的曼尼希碱衍生物，可用作抗癌剂。Autophagy inducer 4 通过诱导自噬 (autophagy) 来抑制癌细胞。Autophagy inducer 4 对 T47D 细胞的毒性是 Magnolol 的 76 倍。Autophagy inducer 4 对 T47D 和 Hela 癌细胞的迁移也有抑制作用。

Autophagy inducer 4

Autophagy inducer 4 Chemical Structure

CAS No. : 2486455-03-0

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生物活性

Autophagy inducer 4 is a Magnolol-based Mannich base derivatives, which can be used as an anticancer agent. Autophagy inducer 4 suppresses cancer cells via inducing autophagy. Autophagy inducer 4 has 76-fold improvement in cytotoxicity against T47D cells compared with Magnolol. Autophagy inducer 4 also possesses suppressive effects on migration of T47D and Hela cancer cells[1].

IC50 & Target

IC50: 0.91 μM in T47D, 3.32 μM in MCF-7, 1.71 μM in Hela[1]
体外研究
(In Vitro)

Autophagy inducer 4 (compound 3p) (0-10 μM; 72 hours) displays highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines[1].
Autophagy inducer 4 (40-80 μM; 0-36 hours) significantly increases GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells[1].
Autophagy inducer 4 (0-80 μM; 0-36 hours) increases the transformation of LC3-I into LC3-II in a dose- and time-dependent manner[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: T47D, MCF-7 and Hela cells[1]
Concentration: 0-10 μM
Incubation Time: 72 hours
Result: Displayed highly potent antiproliferative activity against T47D, MCF-7 and Hela cell lines with IC50 values of 0.91, 3.32 and 1.71 μM, respectively.

Cell Autophagy Assay

Cell Line: GFP-LC3-HEK293[1]
Concentration: 40, 60 and 80 μM
Incubation Time: 0, 12, 24 and 36 hours
Result: Significantly increased GFP-LC3 protein puncta with both dose- and time-dependent manners in HEK293 cells.

Western Blot Analysis

Cell Line: Hela, T47D and HEK293[1]
Concentration: 0, 5, 10 and 20 μM in T47D and Hela; 0, 40, 60 and 80 μM in HEK293
Incubation Time: 0, 12, 24 and 36 hours
Result: Increased the transformation of LC3-I into LC3-II in a dose-dependent manner, and enhanced the expression of LC3-II in a time-dependent manner.

分子量

531.64

Formula

C32H37NO6
CAS 号

2486455-03-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Xu T, et al. Semisynthesis of novel magnolol-based Mannich base derivatives that suppress cancer cells via inducing autophagy. Eur J Med Chem. 2020;205:112663.

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