炔-PEG₄-马来酰亚胺

炔基-PEG 4-马来酰亚胺是一种杂双功能交联剂，能够使含叠氮化物的化合物或生物分子与巯基连接。马来酰亚胺基团将优先与硫醇反应形成共价加合物，并且末端炔烃将通过铜催化的1，3-偶极环加成点击化学与叠氮化物反应。亲水性PEG间隔基增加了该试剂的水溶性，可轻松修饰含有半胱氨酸的生物分子，例如带有末端炔基的抗体，蛋白质和肽。PEG间隔基还通过增加亲水性来减少溶液中存储的修饰蛋白的聚集。

Tris Buffered Saline with Tween 20: 500 ml of 10X is a high-quality 10X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.|Additional sizes and formats available:|Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)|Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)|Tris Buffered Saline with Tween 20: 1 L of 10X (sc-24953)|Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)|Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)|Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)|Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)|Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)|Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)|Also available without Tween 20:|Tris Buffered Saline: 1 L of 1X (sc-362185)|Tris Buffered Saline: 1 L of 10X (sc-24951)|Tris Buffered Saline: 100 ml of 10X (sc-362303)|Tris Buffered Saline: 500 ml of 10X (sc-362304)|Tris Buffered Saline: 1 L of 20X (sc-362186)|Tris Buffered Saline: 5 L of 20X (sc-362305)|Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)|Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)|Tris Buffered Saline: powder for 1 L of 10X (sc-362307)|Tris Buffered Saline: powder for 5 L of 10X (sc-362308)
Dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 5 L of 1X TBST buffer.Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%.
Tris Buffered Saline with Tween 20: 500 ml of 10Xis a convenient prepared buffer for use in various applications