乙炔-PEG₅-NHS酯

乙炔-PEG 5 -NHS酯是一种杂双功能交联剂，可将含叠氮化物的化合物或生物分子与伯胺连接。琥珀酰亚胺基酯基将优先与伯胺反应形成共价加合物，末端炔烃将通过铜催化的1,3-偶极环加成点击化学与叠氮化物反应。亲水性PEG间隔基增加了该试剂的水溶性，使末端胺炔易于修饰含胺的生物分子，例如抗体，蛋白质和肽。PEG间隔基还通过增加亲水性来减少溶液中存储的修饰蛋白的聚集。

Tris Buffered Saline: 5 L of 20X is ready to use for dilution to a 1X working solution (pH 7.4). Tris Buffered Saline (TBS) is a pH stabilizing solution used for western blot and ELISA procedures. It is an optimal formulation of salts and pH stabilizers which enable washing without disruption of antibody-antigen binding interactions.|Additional sizes and formats available:|Tris Buffered Saline: 1 L of 1X (sc-362185)|Tris Buffered Saline: 1 L of 10X (sc-24951)|Tris Buffered Saline: 100 ml of 10X (sc-362303)|Tris Buffered Saline: 500 ml of 10X (sc-362304)|Tris Buffered Saline: 1 L of 20X (sc-362186)|Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)|Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)|Tris Buffered Saline: powder for 1 L of 10X (sc-362307)|Tris Buffered Saline: powder for 5 L of 10X (sc-362308)|Also available with Tween 20:|Tris Buffered Saline with Tween 20:1 L of 1X (sc-362187)|Tris Buffered Saline with Tween 20:100 ml of 10X (sc-362309)|Tris Buffered Saline with Tween 20:500 ml of 10X (sc-362310)|Tris Buffered Saline with Tween 20:1 L of 10X (sc-24953)|Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)|Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)|Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)|Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)|Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)|Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)
Prior to use, dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 100 L of 1X TBS buffer. Store the buffer solution at +4°C. Final (1X) pH 7.4 ±0.2 (25°C), may need slight pH adjustment.pH’: ‘When dilut
Tris Buffered Saline: 5 L of 20Xis a convenient prepared buffer for use in various applications