Thapsigargin(Synonyms: 毒胡萝卜素)

Thapsigargin (Synonyms: 毒胡萝卜素) 纯度: 99.95%

Thapsigargin,内质网应激诱导剂, 是一种微粒体 Ca2+-ATPase 抑制剂。Thapsigargin 能有效抑制不同细胞类型的冠状病毒 (HCoV-229E、MERS-CoV、SARS-CoV-2) 复制。

Thapsigargin(Synonyms: 毒胡萝卜素)

Thapsigargin Chemical Structure

CAS No. : 67526-95-8

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Thapsigargin 相关产品

相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Thapsigargin, an endoplasmic reticulum (ER) stress inducer, is an inhibitor of microsomal Ca2+-ATPase. Thapsigargin efficiently inhibits coronavirus (HCoV-229E, MERS-CoV, SARS-CoV-2) replication in different cell types[1][2][3][4][5].

IC50 & Target

Ca2+-ATPase[1]

体外研究
(In Vitro)

Thapsigargin (0.001- 1 μM; for 2 and 4 days) arrests cell proliferations in MH7A human rheumatoid arthritis synovial cells in a time- and dose-dependent manner[2].
Thapsigargin (0.001- 1 μM; for 2 and 4 days) induces cell apoptosis in MH7A cells in a time- and dose-dependent manner[2].
Thapsigargin (0.001- 1 μM; for 2 and 4 days) impairs mTOR activity and leads to cyclin D1 expressions in MH7A cells[2].
Thapsigargin inhibits Ca2+ entry into human neutrophil granulocytes[1].
Thapsigargin inhibits the carbachol-evoked [Ca2+]i-transients with (IC50=0.353 nM) or without (IC50=0.448 nM) a KCl-prestimulation, but an additional small component, with a much lower sensitivity (IC50=4814 nM), is observed in the absence of a KCl-prestimulation. In contrast, the KCl-evoked [Ca2+]i-transients displayed only one component with a very low sensitivity to Thapsigargin in both absence (IC50=3343 nM) and presence (IC50=6858 nM) of a carbachol-prestimulation[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: MH7A human rheumatoid arthritis synovial cells
Concentration: 0.001, 0.1, and 1 μM
Incubation Time: For 2 and 4 days
Result: Arrested cell proliferations in a time- and dose-dependent manner.

Apoptosis Analysis[2]

Cell Line: MH7A human rheumatoid arthritis synovial cells
Concentration: 0.001, 0.1, and 1 μM
Incubation Time: For 2 and 4 days
Result: Induces cell apoptosis in a time- and dose-dependent manner.

Western Blot Analysis[2]

Cell Line: MH7A human rheumatoid arthritis synovial cells
Concentration: 0.001, 0.1, and 1 μM
Incubation Time: For 2 and 4 days
Result: Impairs mTOR activity and leads to cyclin D1 expressions

体内研究
(In Vivo)

Thapsigargin (Injection; 0.25 ug/g, 0.5 ug/g and 1 ug/g; 24 hours) significant increases of 2 to 5-fold in chemokine and pro-inflammatory expression. Thapsigargin is more sensitive to inducing a systemic immune response[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Balb/c mice (20-25 g)[4]
Dosage: 0.25 ug/g, 0.5 ug/g and 1 ug/g
Administration: Injection; 24 hours
Result: Increased of 2 to 5-fold in chemokine and pro-inflammatory expression.

分子量

650.75

Formula

C34H50O12

CAS 号

67526-95-8

中文名称

毒胡萝卜素;毒胡萝卜内酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL (153.67 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5367 mL 7.6834 mL 15.3669 mL
5 mM 0.3073 mL 1.5367 mL 3.0734 mL
10 mM 0.1537 mL 0.7683 mL 1.5367 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90% saline

    Solubility: 5 mg/mL (7.68 mM); Suspended solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.20 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.20 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Geiszt M, et al. Thapsigargin inhibits Ca2+ entry into human neutrophil granulocytes. Biochem J. 1995 Jan 15;305 ( Pt 2):525-8.

    [2]. Wang H, et al. Effects of thapsigargin on the proliferation and survival of human rheumatoid arthritis synovialcells. ScientificWorldJournal. 2014 Feb 9;2014:605416.

    [3]. Garavito-Aguilar ZV, et al. Differential thapsigargin-sensitivities and interaction of Ca2+ stores in human SH-SY5Y neuroblastoma cells. Brain Res. 2004 Jun 18;1011(2):177-86.

    [4]. Abdullahi A, et al. Modeling Acute ER Stress in Vivo and in Vitro. Shock. 2017 Apr;47(4):506-513.

    [5]. Mohammed Samer Shaban, et al. Inhibiting coronavirus replication in cultured cells by chemical ER stress. bioRxiv 2020.08.26.266304;