DMOG (Synonyms: Dimethyloxallyl Glycine) 纯度: 98.70%
DMOG (Dimethyloxallyl Glycine) 是具有细胞渗透和竞争性 HIF-PH 抑制剂,可导致蛋白 HIF-1α 的积聚和稳定。DMOG 是 α-ketoglutarate 的类似物并且可以抑制 α-KG 依耐性羟化酶。DMOG 是一种促血管生成剂,在结肠炎和腹泻动物模型中起保护作用。DMOG 可以诱导细胞自噬 (autophagy).
![DMOG Chemical Structure DMOG(Synonyms: Dimethyloxallyl Glycine)](http://www.fluoroprobe.com/wp-content/uploads/2022/05/hy-15893.gif)
DMOG Chemical Structure
CAS No. : 89464-63-1
规格 | 价格 | 是否有货 | 数量 |
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Free Sample (0.1-0.5 mg) | Apply now | ||
10 mM * 1 mL in DMSO | ¥715 | In-stock | |
50 mg | ¥650 | In-stock | |
100 mg | ¥990 | In-stock | |
200 mg | ¥1750 | In-stock | |
500 mg | ¥3950 | In-stock | |
1 g | 询价 | ||
5 g | 询价 |
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DMOG 相关产品
•相关化合物库:
- Bioactive Compound Library Plus
- Epigenetics Compound Library
- Immunology/Inflammation Compound Library
- Metabolism/Protease Compound Library
- Anti-Cancer Compound Library
- Autophagy Compound Library
- Reprogramming Compound Library
- Oxygen Sensing Compound Library
- Glutamine Metabolism Compound Library
- Anti-Breast Cancer Compound Library
- Anti-Cancer Metabolism Compound Library
- Angiogenesis Related Compound Library
- Transcription Factor Targeted Library
生物活性 |
DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation in vitro and in vivo[1]. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal[2][4]. DMOG induces cell autophagy[5]. |
IC50 & Target |
HIF-1α prolyl hydroxylase[1] |
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体外研究 (In Vitro) |
DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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体内研究 (In Vivo) |
DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
175.14 |
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Formula |
C6H9NO5 |
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CAS 号 |
89464-63-1 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:
DMSO : ≥ 50 mg/mL (285.49 mM) H2O : 50 mg/mL (285.49 mM; Need ultrasonic) * “≥” means soluble, but saturation unknown. 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
*以上所有助溶剂都可在 MCE 网站选购。
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参考文献 |
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Cell Assay [3] |
To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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Animal Administration [2] |
Two groups of mice (C57Bl6) are used. One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
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