生物活性分子抑制剂Periplocin

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Periplocin  纯度: 99.79%

Periplocin 是一类从 Periploca forrestii 中分离出的强心类固醇。Periplocin 可促进肿瘤细胞凋亡并抑制肿瘤生长。Periplocin 通过激活 Na/K-ATPase 介导的 Src/ERK 和 PI3K/Akt 途径而具有促进伤口愈合的潜力。

Periplocin

Periplocin Chemical Structure

CAS No. : 13137-64-9

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1230 In-stock
5 mg ￥800 In-stock
10 mg ￥1400 In-stock
20 mg ￥2300 In-stock
50 mg   询价  
100 mg   询价  

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生物活性

Periplocin is a cardiotonic steroid isolated from Periploca forrestii. Periplocin promotes tumor cell apoptosis and inhibits tumor growth. Periplocin has the potential to facilitate wound healing through the activation of Src/ERK and PI3K/Akt pathways mediated by Na/K-ATPase[1][2].

IC50 & Target

Apoptosis[2]
体外研究
(In Vitro)

Periplocin (5-20 μM; 48 hours; L929 cells) treatment shows increased proliferation up to 131% at 20 μM[1].
Periplocin (5-20 μM; 30-120 minutes; L929 cells) increases phosphorylation of Src, ERK, PI3K and Akt at active sites in a dosedependent and time-dependent manner. Periplocin (5-20 μM; 48 hours) significantly promotes migration of fibroblast cell[1].
Periplocin (5-20 μM; 48 hours) increases collagen production in L929 fibroblast[1].
Periplocin induces Na/KATPase mediates the activation of Src/ERK and PI3K/Akt pathways[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: L929 cells
Concentration: 5 μM, 10 μM, 20 μM
Incubation Time: 48 hours
Result: Showed increased proliferation up to 131% at 20 μM.

Western Blot Analysis[1]

Cell Line: L929 cells
Concentration: 5 μM, 10 μM, 20 μM
Incubation Time: 30 minutes, 60 minutes, 120 minutes
Result: Increased phosphorylation of Src, ERK, PI3K and Akt at active sites in a dosedependent and time-dependent manner.

体内研究
(In Vivo)

Periplocin (5-20 mg/kg; intraperitoneal injection; daily; for 14 days; female SCID mice) treatment represses the growth of hepatocellular carcinoma (HCC) in xenograft tumor model in mice[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female SCID mice (6-8 weeks old) injected with Huh-7 cells[2]
Dosage: 5 mg/kg, 20 mg/kg
Administration: Intraperitoneal injection; daily; for 14 days
Result: Repressed the growth of hepatocellular carcinoma (HCC) in xenograft tumor model in mice.

分子量

696.82

Formula

C36H56O13
CAS 号

13137-64-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (143.51 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4351 mL 7.1755 mL 14.3509 mL
5 mM 0.2870 mL 1.4351 mL 2.8702 mL
10 mM 0.1435 mL 0.7175 mL 1.4351 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.59 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.59 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.59 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.59 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.59 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.59 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 上海金畔生物科技有限公司 网站选购。
参考文献

  • [1]. Chen L, et al. Periplocin promotes wound healing through the activation of Src/ERK and PI3K/Akt pathways mediated by Na/K-ATPase. Phytomedicine. 2019 Apr;57:72-83.

    [2]. Cheng CF, et al. Antitumor Effect of Periplocin in TRAIL-Resistant Human Hepatocellular Carcinoma Cells through Downregulation of IAPs. Evid Based Complement Alternat Med. 2013;2013:958025.

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