Arctigenin ((-)-Arctigenin), a biologically active lignan, can be used as an antitumor agent. Arctigenin exhibits potent antioxidant, anti-inflammatory and antiviral (influenza A virus) activities. Arctigenin can be used for the research of metabolic disorders, and central nervous system dysfunctions^[1][2][3].

Arctigenin (0.01-1 μg/mL; 0.5-24 h) shows preferential cytotoxicity against the PANC-1 cell line under nutrient-deprived conditions in a concentration- and time-dependent manner^[1].
Arctigenin (0.01-1 μg/mL; 0.5-24 h) inhibits the survival of various cancer cell lines (ASPC-1, BXPC-3, Alexander, and PSN-1 cells) under nutrient-deprived conditions^[1].
Arctigenin (0.1-1 μg/mL; 24 h) completely inhibits Akt phosphorylation stimulated by glucose deprivation in PANC-1 cells^[1].
Arctigenin (5-25 μM) shows potent in vitro antiviral activities (IC₅₀=2.9 μM) against influenza A virus^[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Arctigenin (250 μg/mL; 0.2 mL; i.p. 6 times a week for 9 weeks) suppress the PANC-1 tumor growth in nude mice^[1].
Arctigenin (10 mg/kg; i.p. daily for 30 days) relieves experimental autoimmune encephalomyelitis (EAE) symptoms of mice^[4].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

372.41

C₂₁H₂₄O₆

7770-78-7

牛蒡子苷元；牛蒡苷元；牛蒡甙元

Room temperature in continental US; may vary elsewhere.

DMSO : ≥ 125 mg/mL (335.65 mM)

* “≥” means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 6.25 mg/mL (16.78 mM); Clear solution

  此方案可获得 ≥ 6.25 mg/mL (16.78 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 62.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: ≥ 6.25 mg/mL (16.78 mM); Clear solution

  此方案可获得 ≥ 6.25 mg/mL (16.78 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 62.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 6.25 mg/mL (16.78 mM); Clear solution

  此方案可获得 ≥ 6.25 mg/mL (16.78 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 62.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Awale S, et, al. Identification of arctigenin as an antitumor agent having the ability to eliminate the tolerance of cancer cells to nutrient starvation. Cancer Res. 2006 Feb 1;66(3):1751-7.

  [2]. Gao Q, et, al. Overview of the anti-inflammatory effects, pharmacokinetic properties and clinical efficacies of arctigenin and arctiin from Arctium lappa L. Acta Pharmacol Sin. 2018 May;39(5):787-801.

  [3]. Hayashi K, et, al. Therapeutic effect of arctiin and arctigenin in immunocompetent and immunocompromised mice infected with influenza A virus. Biol Pharm Bull. 2010;33(7):1199-205.

  [4]. Wei L, et, al. Arctigenin Exerts Neuroprotective Effect by Ameliorating Cortical Activities in Experimental Autoimmune Encephalomyelitis In Vivo. Front Immunol. 2021 Jul 19;12:691590.

  [5]. Maxwell T, et, al. Arctigenin inhibits the activation of the mTOR pathway, resulting in autophagic cell death and decreased ER expression in ER-positive human breast cancer cells. Int J Oncol. 2018 Apr;52(4):1339-1349.