Desthiobiotin PEG Maleimide

Desthiobiotin PEG Maleimide:

Desthiobiotin PEG Maleimide is one of Nanocs’ reactive desthiobiotin PEG crosslinkers that can react with thiol (-SH) groups. Maleimide reacts with thiol/sulfhydryl group at neutral pH quckily and efficiently. Reaction between maleimide and thiol allows conjugation of desthiobiotin tag to proteins, antibodies, peptides or nucleic acids with high efficiency. Desthiobiotin is a modified version of biotin. Compared to biotin, desthiobiotin binds to streptavidin, avidin and other biotin-binding proteins with lower affinity and it can be eluted from binding complex under much milder conditions (i.e., by competitive displacement with regular, free biotin). As such, desthiobiotin reagent is a useful alternative to Biotin for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. PEG linker between desthiobiotin and maleimide offers better water solubility, less steric hindrance and enhanced stability.

Physical Properties:

• Off-white/white solid;
• Soluble in water, DMSO or DMF;
• Reactive group: Maleimide;
• Reactive to: Thiol (-SH);

Storage Conditions:

• Store at -20 ⁰C. Desiccate. Avoid frequent thaw and freeze.