体外研究 (In Vitro) |
HNHA (0-100 μM, 96 h) shows strong inhibition at lower concentrations on cancer cell lines, especially on breast cancer cells, mouse FM3A and human MCF-7[1]. HNHA (15 μM, 24 h) arrests cancer cells at the G1/S phase of the cell cycle, activates p21and rescues strongly protein acetylation[1]. HNHA (15 μM, 12 h) inhibits angiogenic proteins in breast cancer cells, effectively inactivates MMP-2, MMP-9, VEGF and HIF-1α[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay
Cell Line: |
FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29[1] |
Concentration: |
0-100 μM |
Incubation Time: |
96 h |
Result: |
Showed strong inhibition at lower concentrations on all cancer cell lines (FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29), with IC50 values of 15.70, 55.63, 22.78, 23.18, 26.70, 19.64, 21.26, 22.31, 65.09, 14.33, and 16.98 μM, respectively. |
Cell Viability Assay
Cell Line: |
FM3A and MCF-7[1] |
Concentration: |
0, 0.1, 1, 5, 10, 15, 20, 25,30 μM |
Incubation Time: |
48 h |
Result: |
Showed dose-dependent inhibition of viability in mouse and human breast cancer cells. |
Cell Cycle Analysis
Cell Line: |
FM3A and MCF-7 cells[1] |
Concentration: |
15 μM |
Incubation Time: |
24 h |
Result: |
Arrested FM3A and MCF-7 cells in the G1/S phase. |
Western Blot Analysis
Cell Line: |
FM3A and MCF-7 cells[1] |
Concentration: |
0, 0.1, 1, 10, and 20 μM (24 h) |
Incubation Time: |
1, 6, 24, 48, and 72 h (15 μM) |
Result: |
Activated a cell proliferation arrestor p21, increased histone and non-histone protein acetylation and inhibited FM3A and MCF-7 proliferation in vitro, and was very effective in increasing the acetylation level of histone H3 protein in FM3A and MCF-7. The most effective dose point for acetylation of histone H3 was 10-20 μM. Histone H3 acetylation peaked after 1 h of exposure to the drugs and remained stable for 1-6 h. |
Western Blot Analysis
Cell Line: |
FM3A and MCF-7 cells[1] |
Concentration: |
15 μM |
Incubation Time: |
12 h |
Result: |
Showed a strong induction of TIMP-1 and TIMP-2, and effectively inactivated MMP-2, MMP-9, VEGF and HIF-1α. |
|
体内研究 (In Vivo) |
HNHA (20 μM/mouse, IP, once every 2 days for a total of six injections) reduces tumor burden and extends the survival rate, activates TIMP-1, TIMP-2 and p21 and inhibits MMP-2, MMP-9, HIF-1α and VEGF protein expression[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model: |
C3H/HeJ-FasL mice (FM3A breast cancer cell tumor xenograft, 6 weeks, n = 25/group)[1] |
Dosage: |
20 μM/mouse |
Administration: |
IP, once every 2 days for a total of six injections |
Result: |
Reduced tumor burden and extended the survival rate. Effectively inhibited cancer development and angiogenesis in vivo. Increased TIMP-1, TIMP-2 and p21, decreased MMP-2, MMP-9, HIF-1α and VEGF protein expression, and reduced the distribution of CD34, HIF-1α and VEGF. |
|