标签归档：escs

Cardiogenol C

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Cardiogenol C 

Cardiogenol C 是一种有效的细胞渗透性嘧啶诱导剂，可促使 ESCs 分化为心肌细胞 (ESCs differentiation into cardiomyocytes) (EC50=100 nM)。Cardiogenol C 还可以在有限的可塑性范围内对已经沿袭的祖细胞类型产生心肌病。Cardiogenol C 是一种有效的心肌发生剂。

Cardiogenol C

Cardiogenol C Chemical Structure

CAS No. : 671225-39-1

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Cardiogenol C 的其他形式现货产品:

Cardiogenol C hydrochloride

生物活性

Cardiogenol C is a potent cell-permeable pyrimidine inducer which prompts the differentiation of ESCs into cardiomyocytes (EC50=100 nM)[1]. Cardiogenol C also acts cardiomyogenic on already lineage-committed progenitor cell types with a limited degree of plasticity. Cardiogenol C is a useful cardiomyogenic agent and can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models[2].

IC50 & Target

EC50: 100 nM (differentiation of ESCs into cardiomyocytes)[1]
体外研究
(In Vitro)

Cardiogenol C (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF, nppa) in P19 cells when it compares to untreated control cells[1].
Cardiogenol C (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells[2].
Cardiogenol C (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells[2].
Cardiogenol C (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM
Incubation Time: 7 days
Result: Increases ANF and NKX2.5 mRNA level as a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: C2C12 cells
Concentration: 0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM
Incubation Time: 7 days
Result: Increased cardiac Nav1.5 sodium channel protein levels.

Cell Proliferation Assay[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM
Incubation Time: 7 days
Result: Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment.

分子量

260.29

Formula

C13H16N4O2
CAS 号

671225-39-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Wu X, et al. Small molecules that induce cardiomyogenesis in embryonic stem cells.J Am Chem Soc. 2004 Feb 18;126(6):1590-1.

    [2]. Mike AK, et al. Small molecule cardiogenol C upregulates cardiac markers and induces cardiac functional properties in lineage-committed progenitor cells. Cell Physiol Biochem. 2014;33(1):205-21.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Cardiogenol C

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Cardiogenol C 

Cardiogenol C 是一种有效的细胞渗透性嘧啶诱导剂，可促使 ESCs 分化为心肌细胞 (ESCs differentiation into cardiomyocytes) (EC50=100 nM)。Cardiogenol C 还可以在有限的可塑性范围内对已经沿袭的祖细胞类型产生心肌病。Cardiogenol C 是一种有效的心肌发生剂。

Cardiogenol C

Cardiogenol C Chemical Structure

CAS No. : 671225-39-1

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Cardiogenol C 的其他形式现货产品:

Cardiogenol C hydrochloride

生物活性

Cardiogenol C is a potent cell-permeable pyrimidine inducer which prompts the differentiation of ESCs into cardiomyocytes (EC50=100 nM)[1]. Cardiogenol C also acts cardiomyogenic on already lineage-committed progenitor cell types with a limited degree of plasticity. Cardiogenol C is a useful cardiomyogenic agent and can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models[2].

IC50 & Target

EC50: 100 nM (differentiation of ESCs into cardiomyocytes)[1]
体外研究
(In Vitro)

Cardiogenol C (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF, nppa) in P19 cells when it compares to untreated control cells[1].
Cardiogenol C (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells[2].
Cardiogenol C (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells[2].
Cardiogenol C (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM
Incubation Time: 7 days
Result: Increases ANF and NKX2.5 mRNA level as a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: C2C12 cells
Concentration: 0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM
Incubation Time: 7 days
Result: Increased cardiac Nav1.5 sodium channel protein levels.

Cell Proliferation Assay[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM
Incubation Time: 7 days
Result: Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment.

分子量

260.29

Formula

C13H16N4O2
CAS 号

671225-39-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Wu X, et al. Small molecules that induce cardiomyogenesis in embryonic stem cells.J Am Chem Soc. 2004 Feb 18;126(6):1590-1.

    [2]. Mike AK, et al. Small molecule cardiogenol C upregulates cardiac markers and induces cardiac functional properties in lineage-committed progenitor cells. Cell Physiol Biochem. 2014;33(1):205-21.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Cardiogenol C

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Cardiogenol C 

Cardiogenol C 是一种有效的细胞渗透性嘧啶诱导剂，可促使 ESCs 分化为心肌细胞 (ESCs differentiation into cardiomyocytes) (EC50=100 nM)。Cardiogenol C 还可以在有限的可塑性范围内对已经沿袭的祖细胞类型产生心肌病。Cardiogenol C 是一种有效的心肌发生剂。

Cardiogenol C

Cardiogenol C Chemical Structure

CAS No. : 671225-39-1

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Cardiogenol C 的其他形式现货产品:

Cardiogenol C hydrochloride

生物活性

Cardiogenol C is a potent cell-permeable pyrimidine inducer which prompts the differentiation of ESCs into cardiomyocytes (EC50=100 nM)[1]. Cardiogenol C also acts cardiomyogenic on already lineage-committed progenitor cell types with a limited degree of plasticity. Cardiogenol C is a useful cardiomyogenic agent and can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models[2].

IC50 & Target

EC50: 100 nM (differentiation of ESCs into cardiomyocytes)[1]
体外研究
(In Vitro)

Cardiogenol C (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF, nppa) in P19 cells when it compares to untreated control cells[1].
Cardiogenol C (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells[2].
Cardiogenol C (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells[2].
Cardiogenol C (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM
Incubation Time: 7 days
Result: Increases ANF and NKX2.5 mRNA level as a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: C2C12 cells
Concentration: 0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM
Incubation Time: 7 days
Result: Increased cardiac Nav1.5 sodium channel protein levels.

Cell Proliferation Assay[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM
Incubation Time: 7 days
Result: Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment.

分子量

260.29

Formula

C13H16N4O2
CAS 号

671225-39-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Wu X, et al. Small molecules that induce cardiomyogenesis in embryonic stem cells.J Am Chem Soc. 2004 Feb 18;126(6):1590-1.

    [2]. Mike AK, et al. Small molecule cardiogenol C upregulates cardiac markers and induces cardiac functional properties in lineage-committed progenitor cells. Cell Physiol Biochem. 2014;33(1):205-21.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务