标签归档：glycine

Glycine-d3

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Glycine-d3 

Glycine-d3 是 Glycine 氘代物。Glycine 是中枢神经系统的一种抑制性神经递质，同时也是 glutamate 的一种联合激动剂，有促进谷氨酸能 NMDA (N-methyl-D-aspartic acid ) 受体兴奋的潜能。

Glycine-d3

Glycine-d3 Chemical Structure

CAS No. : 4896-76-8

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Glycine-d3 is the deuterium labeled Glycine. Glycine is an inhibitory neurotransmitter in the CNS and also acts as a co-agonist along with glutamate, facilitating an excitatory potential at the glutaminergic N-methyl-D-aspartic acid (NMDA) receptors.

体外研究
(In Vitro)

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

78.09

Formula

C2H2D3NO2
CAS 号

4896-76-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216.

    [2]. Johnson JW, et al. Glycine potentiates the NMDA response in cultured mouse brain neurons. Nature. 1987 Feb 5-11;325(6104):529-31.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

DMOG(Synonyms: Dimethyloxallyl Glycine)

发表于由

DMOG (Synonyms: Dimethyloxallyl Glycine) 纯度: 98.70%

DMOG (Dimethyloxallyl Glycine) 是具有细胞渗透和竞争性 HIF-PH 抑制剂，可导致蛋白 HIF-1α 的积聚和稳定。DMOG 是 α-ketoglutarate 的类似物并且可以抑制 α-KG 依耐性羟化酶。DMOG 是一种促血管生成剂，在结肠炎和腹泻动物模型中起保护作用。DMOG 可以诱导细胞自噬 (autophagy).

DMOG(Synonyms: Dimethyloxallyl Glycine)

DMOG Chemical Structure

CAS No. : 89464-63-1

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ￥715 In-stock
50 mg ￥650 In-stock
100 mg ￥990 In-stock
200 mg ￥1750 In-stock
500 mg ￥3950 In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

DMOG 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Epigenetics Compound Library
  • Immunology/Inflammation Compound Library
  • Metabolism/Protease Compound Library
  • Anti-Cancer Compound Library
  • Autophagy Compound Library
  • Reprogramming Compound Library
  • Oxygen Sensing Compound Library
  • Glutamine Metabolism Compound Library
  • Anti-Breast Cancer Compound Library
  • Anti-Cancer Metabolism Compound Library
  • Angiogenesis Related Compound Library
  • Transcription Factor Targeted Library

生物活性

DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation in vitro and in vivo[1]. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal[2][4]. DMOG induces cell autophagy[5].

IC50 & Target

HIF-1α prolyl hydroxylase[1]
体外研究
(In Vitro)

DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

175.14

Formula

C6H9NO5
CAS 号

89464-63-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 50 mg/mL (285.49 mM)

H2O : 50 mg/mL (285.49 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.7097 mL 28.5486 mL 57.0972 mL
5 mM 1.1419 mL 5.7097 mL 11.4194 mL
10 mM 0.5710 mL 2.8549 mL 5.7097 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： PBS

    Solubility: 150 mg/mL (856.46 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Baader E, et al. Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues. Biochem J. 1994 Jun 1;300 (Pt 2):525-30.

    [2]. Milkiewicz M, et al. Inhibition of endogenous HIF inactivation induces angiogenesis in ischaemic skeletal muscles of mice. J Physiol. 2004 Oct 1;560(Pt 1):21-6.

    [3]. Schultz K, et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L921-7.

    [4]. Li X, et al. Up-regulation of hypoxia-inducible factor-1α enhanced the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8.

    [5]. Singh A, et al. Hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors induce autophagy and have a protective effect in an in-vitro ischaemia model.Sci Rep. 2020 Jan 31;10(1):1597.
Cell Assay
[3]

To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Two groups of mice (C57Bl6) are used. One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Baader E, et al. Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues. Biochem J. 1994 Jun 1;300 (Pt 2):525-30.

    [2]. Milkiewicz M, et al. Inhibition of endogenous HIF inactivation induces angiogenesis in ischaemic skeletal muscles of mice. J Physiol. 2004 Oct 1;560(Pt 1):21-6.

    [3]. Schultz K, et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L921-7.

    [4]. Li X, et al. Up-regulation of hypoxia-inducible factor-1α enhanced the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8.

    [5]. Singh A, et al. Hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors induce autophagy and have a protective effect in an in-vitro ischaemia model.Sci Rep. 2020 Jan 31;10(1):1597.

Soybean lectin (Glycine Max), FITC labeled Cat. No. SB1-FC-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), FITC labeled

Cat. No. SB1-FC-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Soybean lectin (Glycine Max), Rhodamine labeled Cat. No. SB1-RB-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), Rhodamine labeled

Cat. No. SB1-RB-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Soybean lectin (Glycine Max), Cy3 labeled Cat. No. SB1-S3-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), Cy3 labeled

Cat. No. SB1-S3-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Soybean lectin (Glycine Max), Cy5 labeled Cat. No. SB1-S5-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), Cy5 labeled

Cat. No. SB1-S5-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.