标签归档：pla

Atg4B-IN-2

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Atg4B-IN-2 

Atg4B-IN-2 是一种有效的竞争性 Atg4B 抑制剂，Ki 为 3.1 μM，也具有降低 PLA2 的抑制能力，对 Atg4B 和 PLA2 的 IC50 分别为 11 μM 和 3.5 μM。Atg4B-IN-2 通过细胞自噬 (autophagy) 来增强抗去势抗性前列腺癌药物的抗癌活性。

Atg4B-IN-2

Atg4B-IN-2 Chemical Structure

CAS No. : 2765008-88-4

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Atg4B-IN-2 is a potent competitive Atg4B inhibitor with Ki value of 3.1 μM, also possesses declining PLA2 inhibitory potency, IC50s of 11 μM and 3.5 μM for Atg4B and PLA2, respectively. Atg4B-IN-2 enhances the anticancer activity of anti-castration-resistant prostate cancer drugs via autophagy inhibition[1].

IC50 & Target

IC50: 11 μM (Atg4B), 3.5 μM (PLA2)[1]
Ki: 3.1 μM (Atg4B)[1]
体外研究
(In Vitro)

Atg4B-IN-2 (compound 21f) (1-10 μM; 2 hours) restores the p62 expression in cells treated with AF (amino acid-free) in a dose-dependent manner[1].
Atg4B-IN-2 (1 and 5 μM; 2 hours) decreases moderately autophagic vesicles at 1 μM, and almost completely inhibits autophagy at 5 μM[1].
Atg4B-IN-2 (5 μM; 2 hours) inhibits Abi-induced autophagy and significantly augments apoptotic cell death and sensitivity to Abi[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line: LNCaP cells (incubated in AF medium)[1]
Concentration: 1, 2, 5, 10 μM
Incubation Time: 2 hours (then incubated in AFM for 24 or 3 hours)
Result: Restored the p62 expression in cells treated with AF in a dose-dependent manner.

Cell Autophagy Assay

Cell Line: LNCaP cells (incubated in AF medium)[1]
Concentration: 1 and 5 μM
Incubation Time: 2 hours (then incubated in AFM for 24 or 3 hours)
Result: Decreased moderately autophagic vesicles at 1 μM, and almost completely inhibited autophagy at 5 μM.

分子量

330.46

Formula

C21H30O3
CAS 号

2765008-88-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kudo Y, Endo S, Fujita M, et al. Discovery and Structure-Based Optimization of Novel Atg4B Inhibitors for the Treatment of Castration-Resistant Prostate Cancer. J Med Chem. 2022;65(6):4878-4892.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Atg4B-IN-2

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Atg4B-IN-2 

Atg4B-IN-2 是一种有效的竞争性 Atg4B 抑制剂，Ki 为 3.1 μM，也具有降低 PLA2 的抑制能力，对 Atg4B 和 PLA2 的 IC50 分别为 11 μM 和 3.5 μM。Atg4B-IN-2 通过细胞自噬 (autophagy) 来增强抗去势抗性前列腺癌药物的抗癌活性。

Atg4B-IN-2

Atg4B-IN-2 Chemical Structure

CAS No. : 2765008-88-4

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Atg4B-IN-2 is a potent competitive Atg4B inhibitor with Ki value of 3.1 μM, also possesses declining PLA2 inhibitory potency, IC50s of 11 μM and 3.5 μM for Atg4B and PLA2, respectively. Atg4B-IN-2 enhances the anticancer activity of anti-castration-resistant prostate cancer drugs via autophagy inhibition[1].

IC50 & Target

IC50: 11 μM (Atg4B), 3.5 μM (PLA2)[1]
Ki: 3.1 μM (Atg4B)[1]
体外研究
(In Vitro)

Atg4B-IN-2 (compound 21f) (1-10 μM; 2 hours) restores the p62 expression in cells treated with AF (amino acid-free) in a dose-dependent manner[1].
Atg4B-IN-2 (1 and 5 μM; 2 hours) decreases moderately autophagic vesicles at 1 μM, and almost completely inhibits autophagy at 5 μM[1].
Atg4B-IN-2 (5 μM; 2 hours) inhibits Abi-induced autophagy and significantly augments apoptotic cell death and sensitivity to Abi[1].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line: LNCaP cells (incubated in AF medium)[1]
Concentration: 1, 2, 5, 10 μM
Incubation Time: 2 hours (then incubated in AFM for 24 or 3 hours)
Result: Restored the p62 expression in cells treated with AF in a dose-dependent manner.

Cell Autophagy Assay

Cell Line: LNCaP cells (incubated in AF medium)[1]
Concentration: 1 and 5 μM
Incubation Time: 2 hours (then incubated in AFM for 24 or 3 hours)
Result: Decreased moderately autophagic vesicles at 1 μM, and almost completely inhibited autophagy at 5 μM.

分子量

330.46

Formula

C21H30O3
CAS 号

2765008-88-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kudo Y, Endo S, Fujita M, et al. Discovery and Structure-Based Optimization of Novel Atg4B Inhibitors for the Treatment of Castration-Resistant Prostate Cancer. J Med Chem. 2022;65(6):4878-4892.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Atg4B-IN-2

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Atg4B-IN-2 

Atg4B-IN-2 是一种有效的竞争性 Atg4B 抑制剂，Ki 为 3.1 μM，也具有降低 PLA2 的抑制能力，对 Atg4B 和 PLA2 的 IC50 分别为 11 μM 和 3.5 μM。Atg4B-IN-2 通过细胞自噬 (autophagy) 来增强抗去势抗性前列腺癌药物的抗癌活性。

Atg4B-IN-2

Atg4B-IN-2 Chemical Structure

CAS No. : 2765008-88-4

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

生物活性

Atg4B-IN-2 is a potent competitive Atg4B inhibitor with Ki value of 3.1 μM, also possesses declining PLA2 inhibitory potency, IC50s of 11 μM and 3.5 μM for Atg4B and PLA2, respectively. Atg4B-IN-2 enhances the anticancer activity of anti-castration-resistant prostate cancer drugs via autophagy inhibition[1].

IC50 & Target

IC50: 11 μM (Atg4B), 3.5 μM (PLA2)[1]
Ki: 3.1 μM (Atg4B)[1]
体外研究
(In Vitro)

Atg4B-IN-2 (compound 21f) (1-10 μM; 2 hours) restores the p62 expression in cells treated with AF (amino acid-free) in a dose-dependent manner[1].
Atg4B-IN-2 (1 and 5 μM; 2 hours) decreases moderately autophagic vesicles at 1 μM, and almost completely inhibits autophagy at 5 μM[1].
Atg4B-IN-2 (5 μM; 2 hours) inhibits Abi-induced autophagy and significantly augments apoptotic cell death and sensitivity to Abi[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line: LNCaP cells (incubated in AF medium)[1]
Concentration: 1, 2, 5, 10 μM
Incubation Time: 2 hours (then incubated in AFM for 24 or 3 hours)
Result: Restored the p62 expression in cells treated with AF in a dose-dependent manner.

Cell Autophagy Assay

Cell Line: LNCaP cells (incubated in AF medium)[1]
Concentration: 1 and 5 μM
Incubation Time: 2 hours (then incubated in AFM for 24 or 3 hours)
Result: Decreased moderately autophagic vesicles at 1 μM, and almost completely inhibited autophagy at 5 μM.

分子量

330.46

Formula

C21H30O3
CAS 号

2765008-88-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kudo Y, Endo S, Fujita M, et al. Discovery and Structure-Based Optimization of Novel Atg4B Inhibitors for the Treatment of Castration-Resistant Prostate Cancer. J Med Chem. 2022;65(6):4878-4892.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Palmitic acid PEG, mPEG-PLA Cat. No. PG1-PLA-5k 5000 Da 100 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Palmitic acid PEG, mPEG-PLA

Cat. No. PG1-PLA-5k Palmitic acid PEG, mPEG-PLA Cat. No. PG1-PLA-5k 5000 Da 100 mg
Specification 5000 Da
Unit Size 100 mg
Price $325.00

Qty Add to Cart

Description:

Palmitic acid is a 16 carbon saturated fatty acid lipid with excellent hydrophobicity. PEG modified palmitic acid is an excellent amphilphilic polymer with both hydrophilicity and hydrophobility. Pegylated lipids are excellent liposome formation materials that can be used for drug delivery, gene transfection and vaccine delivery as well. These materials can also be used for targeted drug delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Palmitic acid PEG, mPEG-PLA Cat. No. PG1-PLA-2k 2000 Da 100 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Palmitic acid PEG, mPEG-PLA

Cat. No. PG1-PLA-2k Palmitic acid PEG, mPEG-PLA Cat. No. PG1-PLA-2k 2000 Da 100 mg
Specification 2000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

Description:

Palmitic acid is a 16 carbon saturated fatty acid lipid with excellent hydrophobicity. PEG modified palmitic acid is an excellent amphilphilic polymer with both hydrophilicity and hydrophobility. Pegylated lipids are excellent liposome formation materials that can be used for drug delivery, gene transfection and vaccine delivery as well. These materials can also be used for targeted drug delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

棕榈酸 PEG, mPEG-PLA, ,Palmitic acid PEG, mPEG-PLA,

发表于由

棕榈酸 PEG, mPEG-PLA

MW 2000 Da

有货

棕榈酸 PEG, mPEG-PLA,  ,Palmitic acid PEG, mPEG-PLA,

品牌：Jinpan
Palmitic acid PEG, mPEG-PLA

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
P164140-100mg 100mg 现货 棕榈酸 PEG, mPEG-PLA,  ,Palmitic acid PEG, mPEG-PLA,  
P164140-500mg 500mg 现货 棕榈酸 PEG, mPEG-PLA,  ,Palmitic acid PEG, mPEG-PLA,  

基本信息

产品名称 棕榈酸 PEG, mPEG-PLA
英文名称 Palmitic acid PEG, mPEG-PLA
规格或纯度 MW 2000 Da
运输条件 超低温冰袋运输

一般描述

Palmitic acid is a 16 carbon saturated fatty acid lipid with excellent hydrophobicity. PEG modified palmitic acid is an excellent amphilphilic polymer with both hydrophilicity and hydrophobility. Pegylated lipids are excellent liposome formation materials that can be used for drμg delivery, gene transfection and vaccine delivery as well. These materials can also be used for targeted drμg delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

Palmitic acid is a 16 carbon saturated fatty acid lipid with excellent hydrophobicity. PEG modified palmitic acid is an excellent amphilphilic polymer with both hydrophilicity and hydrophobility. Pegylated lipids are excellent liposome formation materials that can be used for drug delivery, gene transfection and vaccine delivery as well. These materials can also be used for targeted drug delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

相关属性

敏感性 对光线敏感
溶解性 Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
储存温度 避光,-20°C储存
品牌 Jinpan

棕榈酸 PEG, mPEG-PLA, ,Palmitic acid PEG, mPEG-PLA,

发表于由

棕榈酸 PEG, mPEG-PLA

MW 5000 Da

有货

棕榈酸 PEG, mPEG-PLA,  ,Palmitic acid PEG, mPEG-PLA,

品牌：Jinpan
Palmitic acid PEG, mPEG-PLA

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
P164141-100mg 100mg 现货 棕榈酸 PEG, mPEG-PLA,  ,Palmitic acid PEG, mPEG-PLA,  
P164141-500mg 500mg 现货 棕榈酸 PEG, mPEG-PLA,  ,Palmitic acid PEG, mPEG-PLA,  

基本信息

产品名称 棕榈酸 PEG, mPEG-PLA
英文名称 Palmitic acid PEG, mPEG-PLA
规格或纯度 MW 5000 Da
运输条件 超低温冰袋运输

一般描述

Palmitic acid is a 16 carbon saturated fatty acid lipid with excellent hydrophobicity. PEG modified palmitic acid is an excellent amphilphilic polymer with both hydrophilicity and hydrophobility. Pegylated lipids are excellent liposome formation materials that can be used for drμg delivery, gene transfection and vaccine delivery as well. These materials can also be used for targeted drμg delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

Palmitic acid is a 16 carbon saturated fatty acid lipid with excellent hydrophobicity. PEG modified palmitic acid is an excellent amphilphilic polymer with both hydrophilicity and hydrophobility. Pegylated lipids are excellent liposome formation materials that can be used for drug delivery, gene transfection and vaccine delivery as well. These materials can also be used for targeted drug delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.

相关属性

敏感性 对光线、湿度敏感
溶解性 >10 mg/mL in hot water, chloroform, ethanol, etc.
储存温度 避光,-20°C储存,充氩
品牌 Jinpan
备注 500mg卖完停产，不再备货

Melittin(Synonyms: 蜂毒肽)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Melittin (Synonyms: 蜂毒肽)

Melittin 是一种PLA2激活剂，可刺激低分子量 PLA2 的活性，而不会提高高分子量 PLA2 的活性。

Melittin(Synonyms: 蜂毒肽)

Melittin Chemical Structure

CAS No. : 20449-79-0

规格 价格 是否有货
5 mg ￥1500 询问价格 & 货期
10 mg ￥2600 询问价格 & 货期
25 mg ￥5200 询问价格 & 货期

* Please select Quantity before adding items.

Melittin 的其他形式现货产品:

Melittin TFA

生物活性

Melittin is a PLA2 activator, stimulates the activity of the low molecular weight PLA2, while it does not the increase activity of the high molecular weight PLA2[1][2].

IC50 & Target

PLA2[1]
体外研究
(In Vitro)

Melittin, an immunologically related PLA2 stimulating peptide from bee venom, increases the activity of the high molecular weight enzyme[1]. Melittin is a cytotoxic peptide from bee venom. Melittin exhibits toxicity against both A2780CR and A2780 cells, with IC50 values of 4.5 and 6.8 μg/mL, respectively. Melittin has natural anti-bacterial, anti-viral, and anti-inflammatory properties. It has also been shown to have diverse anticancer effects in several different cancer cell lines including those of gastric, breast, ovarian, liver, prostate, cervical, and lung origins. The mechanisms by which Melittin, an amphipathic haemolytic peptide, exerts its potential anticancer effects include inhibition of cell proliferation, induction of apoptosis, and direct necrosis. Melittin can also prevent EGF-induced cell invasion through its inhibition of the PI3K/Akt/mTOR signaling pathway, but this is primarily related to breast cancer cells[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2846.46

Formula

C131H229N39O31
CAS 号

20449-79-0
Sequence

Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH2
Sequence Shortening

GIGAVLKVLTTGLPALISWIKRKRQQ-NH2
中文名称

蜂毒肽；蜂毒素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Steiner MR, et al. Responses of purified phospholipases A2 to phospholipase A2 activating protein (PLAP) and Melittin. Biochim Biophys Acta. 1993 Feb 10;1166(1):124-30.

    [2]. Alonezi S, et al. Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology. Metabolites. 2016 Oct 13;6(4). pii: E35.
Cell Assay
[2]

Melittin is purified from bee venom by reversed phase liquid chromatography and reconstituted in sterile water to form a stock solution of 1 mg/mL before storage at -20 °C until required for analysis. Cell viability is assessed by an Alamar Blue (AB) cell viability reagent. Both A2780 and A2780CR cells are seeded at 1×104 cells/well in 96-well plates and incubated at 37 °C and 5% CO2 in a humidified atmosphere for 24 h. After this incubation period, the cells are treated with various concentrations of Melittin ranging from 0.5 to 14 µg/mL in 100 μL of medium, and re-incubated at 37 °C and 5% CO2 for a further 24 h. Triton X at 1% (v/v) and cell culture media are used as positive and negative controls, respectively. After this, AB is added at a final concentration of 10% (v/v) and the resultant mixture is incubated for a further 4 h at 37 °C and 5% CO2. Then, the plates are read at an excitation wavelength of 560 nm and the emission at 590 nm is recorded on a SpectraMax M3 microplate reader . Background-corrected fluorescence readings are converted to cell viability data for each test well by expressing them as percentages relative to the mean negative control value[2].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Steiner MR, et al. Responses of purified phospholipases A2 to phospholipase A2 activating protein (PLAP) and Melittin. Biochim Biophys Acta. 1993 Feb 10;1166(1):124-30.

    [2]. Alonezi S, et al. Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology. Metabolites. 2016 Oct 13;6(4). pii: E35.

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务