Pterostilbene is a stilbenoid isolated from blueberries and Pterocarpus marsupium^[1]. Shows anti-oxidant, anti-inflammatory, anti-carcinogenic, anti-diabetic and anti-obesity properties^[1][4]. Pterostilbene blocks ROS production^[3], also exhibits inhibitory activity against various free radicals such as DPPH, ABTS, hydroxyl, superoxide and hydrogen peroxide^[4].

Pterostilbene (0, 5, 25, 50, 100, 200 and 400 µM) shows inhibitory activity against the growth of HeLa cells, with IC₅₀s of 101.2 µM and 65.9 µM at 24 and 48 hrs, respectively. Ipterostilbene (0, 25, 100 and 200 µM) also induces the apoptosis HeLa cells^[2].
Pterostilbene (0.05, 0.1, 0.15 and 0.2 mM) has high anti-oxidant activity against DPPH, ABTS, hydroxyl, superoxide, hydrogen peroxide in a dose-dependent manner. Pterostilbene decreases lipid peroxides and hydroperoxides, reduces protein carbonyl groups and restores protein sulphydryl groups in response to damage by TBHP and As-Fe²⁺. Pterostilbene also inhibits single strand breaks in pBR322^[4].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

Pterostilbene (30 mg/kg daily, p.o. for 21 days) inhibits reactive oxygen species production in the animal model of inflammation^[3].

上海金畔生物科技有限公司 has not independently confirmed the accuracy of these methods. They are for reference only.

256.30

C₁₆H₁₆O₃

537-42-8

紫檀茋

Room temperature in continental US; may vary elsewhere.

DMSO : 110 mg/mL (429.18 mM; Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 3.93 mg/mL (15.33 mM); Clear solution

  此方案可获得 ≥ 3.93 mg/mL (15.33 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 39.3 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.75 mg/mL (10.73 mM); Clear solution

  此方案可获得 ≥ 2.75 mg/mL (10.73 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.75 mg/mL (10.73 mM); Clear solution

  此方案可获得 ≥ 2.75 mg/mL (10.73 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. McCormack D, et al. A review of pterostilbene antioxidant activity and disease modification. Oxid Med Cell Longev. 2013;2013:575482.

  [2]. Hong Bin W, et al. Pterostilbene (3′,5′-dimethoxy-resveratrol) exerts potent antitumor effects in HeLa human cervical cancer cells via disruption of mitochondrial membrane potential, apoptosis induction and targeting m-TOR/PI3K/Akt signalling pathway. J BUON. 2018 Sep-Oct;23(5):1384-1389.

  [3]. Perecko T, et al. The effects of pterostilbene on neutrophil activity in experimental model of arthritis. Biomed Res Int. 2013;2013:106041.

  [4]. Acharya JD, et al. Protective effect of Pterostilbene against free radical mediated oxidative damage. BMC Complement Altern Med. 2013 Sep 26;13:238.