嘌呤霉素 二盐酸盐 来源于白色链球菌
基本信息
| 产品名称 | 嘌呤霉素 二盐酸盐 来源于白色链球菌 |
|---|---|
| 英文名称 | Puromycin dihydrochloride from Streptomyces alboniger |
| 别名 | 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine 二盐酸盐 |
| 英文别名 | 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride |
| 规格或纯度 | 粉末，生物制剂，适用于细胞培养 |
| 运输条件 | 超低温冰袋运输 |
| 生化机理 | Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center. 作用机制：嘌呤霉素通过提前终止链来抑制蛋白质合成，作为氨基酰基-tRNA 的 3′ 末端的类似物起作用。 耐药性机制：嘌呤霉素乙酰转移酶是有效的抗性基因。 抗菌谱：该产品对革兰氏阳性微生物有活性，对耐酸杆菌的活性较低，对革兰氏阴性微生物的活性更低。 嘌呤霉素可以防止细菌、原生动物、藻类和哺乳动物细胞的生长，并且起效迅速，可在 2 天内杀死 99％ 的细胞。 |
一般描述
制备说明
This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.
General Description
Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger . It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes. Chemical structure: peptidyl nucleoside
Application
Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger . It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL. Puromycin dihydrochloride from Streptomyces alboniger has been used: in the preparation of puromycin stock solution for puromycin sensitivity assay. to select lentivirus-infected cells containing puromycin resistant selection marker. Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.
Other Notes
Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place
相关属性
| CAS编号 | 58-58-2 |
|---|---|
| 熔点 | 174°C(lit.) |
| 溶解性 | H2O：可溶 50mg/mL，澄清，无色至淡黄色 |
| 储存温度 | -20°C储存 |
| Reaxys-RN | 3853613 |
| RTECS | AU7355000 |
| 分子量 | 544.44 |
| 分子式 | C22H29N7O5·2HCl |
| EC号 | 200-387-8 |
| 品牌 | Jinpan |
| 备注 | 注意: As supplied, this product remains active for four years when stored at -20°C. |
| 关联CAS | 53-79-2 |
| PubChem CID | 443311 |