Harringtonolide

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上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白，专注于信号通路和疾病研究领域。

Harringtonolide 

Harringtonolide 是一种有效的 RACK1 抑制剂，在 A375 细胞中 IC50=39.66 μM。Harringtonolide 通过影响 FAK 和 RACK1 的相互作用来抑制上皮-间充质转化 (EMT) 过程和细胞增殖。Harringtonolide 具有抑制植物生长、抗病毒、抗炎、抗增殖活性。

Harringtonolide

Harringtonolide Chemical Structure

CAS No. : 64761-48-4

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生物活性

Harringtonolide is a potent RACK1 inhibitor (IC50=39.66 μM in A375 cells). Harringtonolide inhibits the epithelial-mesenchymal transition (EMT) process and cell proliferation by affecting the interaction between FAK and RACK1. Harringtonolide has plant growth inhibitory, antiviral, anti-inflammatory, and antiproliferation activities[1].

IC50 & Target

IC50: 39.66 μM (RACK1) in A375 cells[1]
体外研究
(In Vitro)

Harringtonolide (0-50 μM; 24 hours) exhibits good antiproliferation activity in A375 cells with IC50 of 39.66 μM[1].
Harringtonolide (0-20 μM; 1 hour) restrains the proteolysis of RACK1 by Pronase, and protects temperature-dependent degradation of RACK1[1].
Harringtonolide (0-4 μM; 24 hours) suppresses the phosphorylation of FAK dose-dependently and inhibits Src and STAT3, the downstream proteins of FAK[1].
Harringtonolide (0-4 μM; 24 hours) dose-dependently inhibits the RACK1–FAK interaction in A375 cells[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: A375 melanoma cells[1]
Concentration: 0-50 μM
Incubation Time: 24 hours
Result: Showed good antiproliferation activity with IC50 of 39.66 μM.

Western Blot Analysis

Cell Line: A375 melanoma cells[1]
Concentration: 0, 0.5, 1, 2, 4 μM
Incubation Time: 24 hours
Result: Suppressed the phosphorylation of FAK dose-dependently and inhibited Src and STAT3, the downstream proteins of FAK.

分子量

310.34

Formula

C19H18O4
CAS 号

64761-48-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zhu TY, et al. Photoaffinity Probe Reveals the Potential Target of Harringtonolide for Cancer Cell Migration Inhibition. ACS Med Chem Lett. 2022;13(3):449-456. Published 2022 Feb 2.

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