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Topoisomerase I/II inhibitor 2
Topoisomerase I/II inhibitor 2 (compound 1a) 是一种有效的拓扑异构酶 (Topoisomerase) 抑制剂,Huh7 与 LM9 细胞中的 IC50 分别为 9.82 μM 和 6.83 μM。Topoisomerase I/II inhibitor 2 对 DNA 拓扑异构酶 I/II 具有双重抑制,也能抑制小鼠模型中的异种移植肿瘤。Topoisomerase I/II inhibitor 2 具有治疗肝癌的潜在价值。
Topoisomerase I/II inhibitor 2 Chemical Structure
规格 |
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是否有货 |
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100 mg |
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询价 |
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250 mg |
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询价 |
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500 mg |
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询价 |
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* Please select Quantity before adding items.
生物活性 |
Topoisomerase I/II inhibitor 2 (compound 1a) is a potent Topoisomerase inhibitor (IC50= 9.82 μM on Huh7 cells and 6.83 μM on LM9 cells). Topoisomerase I/II inhibitor 2 has dual inhibition on DNA topoisomerase I/II, also can obviously reduce the growth of xenograft tumor in mice model. Topoisomerase I/II inhibitor 2 has the potential value in treating liver cancer[1].
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IC50 & Target |
Topoisomerase I
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Topoisomerase II
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体外研究 (In Vitro) |
Topoisomerase I/II inhibitor 2 (compound 1a) (0-150 μM; 72 hours) has favourable anti-proliferative activity in cancer cell lines, and better inhibitory activity on two human hepatocellular carcinoma cell lines (HuH7, LM9)[1]. Topoisomerase I/II inhibitor 2 (20 μM; 24 hours) has no damage to the DNA of HuH7 cells while some damage is noticed on LM9 cells[1]. Topoisomerase I/II inhibitor 2 (1.25-8 μM; 1-2 weeks) inhibits cell proliferation of LM9 and Huh7 in a concentration-dependent manner[1]. Topoisomerase I/II inhibitor 2 (1.25-8 μM; 24 hours) has a good inhibitory effect on the migration and invasion of LM9 and HuH7 cells with concentration-dependent manner[1]. Topoisomerase I/II inhibitor 2 (0-20 μM; 24 hours) can inhibit the expression of matrix metalloproteinases-9 (MMP-9) in LM9 and HuH7 cells[1]. Topoisomerase I/II inhibitor 2 (0-20 μM; 48 hours) inhibits cells proliferation by blocking cell cycle at the G2/M phase[1]. Topoisomerase I/II inhibitor 2 (3.5-20 μM; 48 hours) can injure mitochondrial function and induce cell apoptosis in a concentration-dependent manner[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay
Cell Line: |
LM9, HuH7, SK-hep-1, HepG2, HT-29, HCT-116, RKO, SW480, MCF-7, MDA-B-231, HGC-27, SGC-7901, BGC-823, A549, U251, HL-60, LO2[1] |
Concentration: |
0-150 μM |
Incubation Time: |
72 hours |
Result: |
Displayed favourable anti-proliferative activity and had better inhibitory activity on two human hepatocellular carcinoma cell lines (HuH7, LM9). |
Western Blot Analysis
Cell Line: |
LM9 and HuH7 cells[1] |
Concentration: |
0, 3.75, 7.5, 15 μM in LM9; 0, 5, 10, 20 μM in HuH7 |
Incubation Time: |
48 hours |
Result: |
Inhibited the expression of MMP-9. |
Cell Cycle Analysis
Cell Line: |
LM9 and HuH7 cells[1] |
Concentration: |
0, 3.75, 7.5, 15 μM in LM9; 0, 5, 10, 20 μM in HuH7 |
Incubation Time: |
48 hours |
Result: |
Inhibited cells proliferation by blocking cell cycle at the G2/M phase. |
Apoptosis Analysis
Cell Line: |
LM9 and HuH7 cells[1] |
Concentration: |
3.5, 7, 14 μM in LM9; 5, 10, 20 μM in HuH7 |
Incubation Time: |
48 hours |
Result: |
Induced apoptosis in a dose-dependent manner. |
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分子量 |
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Formula |
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运输条件 |
Room temperature in continental US; may vary elsewhere.
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储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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参考文献 |
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