MIRA-1(Synonyms: NSC 19630)

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MIRA-1 (Synonyms: NSC 19630)

MIRA-1 是一种马来酰亚胺类似物。MIRA-1 可通过恢复 p53 依赖的转录转激活诱导 p53 突变细胞凋亡 (apoptosis)。MIRA-1 具有抗肿瘤活性。

MIRA-1(Synonyms: NSC 19630)

MIRA-1 Chemical Structure

CAS No. : 72835-26-8

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生物活性

MIRA-1 is a maleimide analogue. MIRA-1 can induce apoptosis in mutant p53 cells via restoration of p53-dependent transcriptional transactivation. MIRA-1 has anticancer activity[1].

IC50 & Target

IC50: 10 μM (Saos-2 His273)[1]
体外研究
(In Vitro)

MIRA-1 suppresses growth of Saos-2-His273 cells expressing mutant p53[1].
MIRA-1 (25 μM; 48 hours) inhibits cell growth in a mutant p53-dependent manner[1].
MIRA-1 (5 μM; 14 days) dramatically reduces the number of colonies formed by His273-expressing Saos-2 cells, but is much less efficient in inhibiting p53-null Saos-2 cells[1].
MIRA-1 (10 μM; 48 hours) causes a substantial increase in the fraction of Saos-2 and Saos-2-His273 cells with a sub-G1 DNA content in the presence of mutant p53[1].
MIRA-1 (5 and 10 μM; 24 hours) induces EGFP expression, MDM2 and Bax in SKOV-His175 cells[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: Saos-2-His273, H1299-His175, SKOV-His175 and SKOV-His273[1]
Concentration: 25 μM
Incubation Time: 48 hours
Result: Inhibited cell growth in a mutant p53-dependent manner, with survival rate of 18.5~39% in no doxycycline and 71.5~87.5% in doxycycline.

Apoptosis Analysis

Cell Line: Saos-2 and Saos-2-His273 cells[1]
Concentration: 10 μM
Incubation Time: 48 hours
Result: Caused a substantial increase in the fraction of cells with a sub-G1 DNA content in the presence of mutant p53.

分子量

183.16

Formula

C8H9NO4
CAS 号

72835-26-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Bykov VJ, et al. Reactivation of mutant p53 and induction of apoptosis in human tumor cells by maleimide analogs [published correction appears in J Biol Chem. 2017 Dec 1;292(48):19607]. J Biol Chem. 2005;280(34):30384-30391.

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